微生物发酵法生产L-异亮氨酸的研究进展  

Research progress of L-isoleucine production by microbial fermentation

作　　者：孔帅 方应浩 周航 许鹏飞 杨潇 任立伟[1,2,3] 龚大春 KONG Shuai;FANG Yinghao;ZHOU Hang;XU Pengfei;YANG Xiao;REN Liwei;GONG Dachun(School of Biology and Pharmacy,China Three Gorges University,Yichang 443002,China;Hubei Engineering Research Center for Biological Jiaosu,China Three Gorges University,Yichang 443002,China;Key Laboratory of Functional Yeast of China National Light Industry,China Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002 [2]三峡大学湖北省生物酵素工程技术研究中心,湖北宜昌443002 [3]三峡大学中国轻工业功能酵母重点实验室,湖北宜昌443002

出　　处：《中国酿造》2020年第9期30-36,共7页China Brewing

基　　金：国家自然基金项目(21776162);湖北省技术创新专项(No.2019ABA114)。

摘　　要：L-异亮氨酸作为三大支链氨基酸之一,在食品、牲畜饲料和医药等领域都有广泛应用。微生物发酵法是目前生产L-异亮氨酸最主要的方法,选育优良的生产菌种和优化发酵控制工艺有助于提高L-异亮氨酸的产量。该文对L-异亮氨酸的生物合成途径及代谢调控机制、L-异亮氨酸产生菌的选育现状以及发酵控制工艺进行了系统综述,为后续利用微生物发酵法生产L-异亮氨酸的生产研究提供了参考。As one of the three branched chain amino acids,L-isoleucine is widely used in the fields of food,livestock feed and medicine.Microbial fermentation method was currently the most important method for L-isoleucine production,breeding excellent production strains and optimizing fermentation control technology can help increase the production of L-isoleucine.In this paper,the biosynthetic pathway and metabolic regulation mechanism of L-isoleucine,the breeding status of L-isoleucine-producing strain,and the fermentation control process were systematically reviewed,which provided a reference for the follow-up research on the production of L-isoleucine by microbial fermentation method.

关 键 词：L-异亮氨酸 生物合成 菌种选育 发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]