超声辅助纤维素酶法制备杏鲍菇蛋白质工艺优化  

Optimization of Protein Preparation of Pleurotus eryngii by Ultrasonic Assisted Cellulase

作　　者：许新月 崔文玉 黄泽天 弓志青[1] 王文亮[1] 王延圣[1] XU Xin-yue;CUI Wen-yu;HUANG Ze-tian;GONG Zhi-qing;WANG Wen-liang;WANG Yan-sheng(Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China;Agricultural and Rural Bureau of Yanggu County,Liaocheng City,Liaocheng 252300,Shandong,China)

机构地区：[1]山东省农业科学院农产品研究所,山东济南250100 [2]聊城市阳谷县农业农村局,山东聊城252300

出　　处：《食品研究与开发》2020年第20期108-114,共7页Food Research and Development

基　　金：山东省自然科学基金(三院联合基金)(ZR2016YL029);山东省农业科学院青年科研基金(2016YQN48);山东省食用菌创新团队产后加工岗位专家(SDAIT-07-08)。

摘　　要：为提高杏鲍菇蛋白提取率,采用超声波破碎辅助纤维素酶法水解杏鲍菇子实体,制备杏鲍菇蛋白。在单因素试验的基础上,选择料液比、超声功率、超声时间和纤维素酶添加量为影响因素,以蛋白质提取率为指标,进行四因素三水平响应面优化,得到杏鲍菇蛋白质最佳提取工艺,确定影响杏鲍菇蛋白提取率的因素分别为:料液比>超声时间>纤维素酶添加量>超声功率,得到最佳提取条件为:料液比1∶30(g/mL)、超声功率400 W、超声时间20 min、纤维素酶添加量2%。实际提取率75.7%,理论提取率75.1%,相对偏差0.79%。In order to improve the protein extraction rate of Pleurotus eryngii,the seedlings of Pleurotus eryngii were prepared by ultrasonic disruption and cellulase hydrolysis.On the basis of single factor experiment,the ratio of material to liquid,ultrasonic power,ultrasonic time and cellulase was selected as the influencing factors,and the protein extraction rate was used to optimize the four factors and three levels of response surface to obtain the most protein of Pleurotus eryngii.The optimum extraction factors for determining the extraction rate of Pleurotus eryngii were:material-liquid ratio>ultrasonic time>cellulase addition amount>ultrasonic power,and the best extraction conditions were:ratio of material to liquid 1∶30(g/mL),the ultrasonic power was 400 W,the ultrasonic time was 20 min,and the cellulase addition amount was 2%.The actual extraction rate was 75.7%,and the theoretical extraction rate was 75.1%,and the relative deviation was 0.79%.

关 键 词：杏鲍菇 超声辅助 纤维素酶 蛋白提取率 

分 类 号：TQ936[化学工程]