探索黄芪,南、北沙参治疗尿路上皮癌的潜在靶点及作用机制  

To Explore the Potential Target and Mechanism of Astragalus Membranaceus Radix Sachariae Nanensis and Radix Pseudoginsengin the Treatment of Urothelial Carcinoma

作　　者：郑新龙 郑素雅 林根[3] ZHENG Xinlong;ZHENG Suya;LIN Gen(Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Fuzhou Second Hospital,Fuzhou,Fujian 350007,China;Fujian Cancer Hospital,Fuzhou,Fujian 350014,China)

机构地区：[1]福建中医药大学,福建福州350122 [2]福州市第二医院,福建福州350007 [3]福建省肿瘤医院,福建福州350014

出　　处：《大医生》2020年第10期8-11,共4页Doctor

摘　　要：目的探索研究黄芪-南沙参-北沙参(HNB)扶正抗癌中药治疗尿路上皮癌(UC)患者可能的潜在分子机制。方法收集HNB的活性化合物并确定其靶标。分析免疫治疗尿路上皮癌细胞有效和无效的差异表达基因,获得UC免疫疗效相关靶标,构建蛋白互作网络(PPI),并将HNB靶标和UC免疫治疗相关靶标的PPI网络合并并可视化。使用DAVID数据库做GO和KEGG(京都基因与基因组百科全书)分析。构建通路网络以筛选关键靶基因。结果共鉴定30个HNB活性化合物和196个靶标。分析818个差异表达基因。最终确定HNB结合免疫抑制剂治疗UC相关的75个靶基因。发现目标基因与癌症中PD-L1(程序性死亡受体-配体)表达和PD-1(程序性死亡受体)检查点途径、先天免疫的激活、B细胞受体信号通路及T细胞受体信号通路、转化生长因子β受体信号通路以及TGF-beta(转化生长因子β)信号通路相关。结论HNB可能具有免疫激活和减弱免疫抑制的功能。Objective To explore the potential molecular mechanism of Huangqi nanshashen Beishashen(HNB)Jindian Fuzheng anticancer Chinese medicine in the treatment of UC patients.Methods The active compounds of HNB were collected and their targets were determined.We analyzed the differentially expressed genes between the effective and ineffective immunotherapy patients,obtained the targets related to the immune efficacy of UC,constructed the protein interaction network(PPI),and combined and visualized the PPI network of HNB target and UC immunotherapy target.Using David database to do go and KEGG analysis.Construction of pathway network to screen key target genes.Results ThirtyHNB active compounds and 196 targets were identified.818 differentially expressed genes.Finally,75 target genes related to the treatment of UC by HNB combined with immunosuppressant were identified.It was found that the target gene was related to the expression of PD-L1 and PD-1 checkpoint pathway,innate immune activation,B cell receptor signaling pathway,T cell receptor signaling pathway,transforming growth factorβreceptor signaling pathway and TGF beta signaling pathway.Conclusion HNB may have the function of immunoactivation and immunosuppression.

关 键 词：PD-L1 免疫激活 TGF-BETA 扶正中药 

分 类 号：R73[医药卫生—肿瘤]