超声辅助提取紫贻贝加工下脚料抗菌肽工艺优化  

Optimization of ultrasound-assisted extraction process of antimicrobial peptides from the by-products of Mytilus edulis

作　　者：张玥 杨玉霞 时光宇 赵莘芷 潘渊博 徐琼怡 ZHANG Yue;YANG Yu-xia;SHI Guang-yu;ZHAO Xin-zhi;PAN Yuan-bo;XU Qiong-yi(Donghai Science and Technology College,Zhejiang Ocean University,Zhoushan,Zhejiang 316000,China)

机构地区：[1]浙江海洋大学东海科学技术学院,浙江舟山316000

出　　处：《食品与机械》2020年第10期159-164,共6页Food and Machinery

基　　金：浙江省自然科学基金(编号:LY19C200007);国家级大学生创新创业训练计划项目(编号:201810340038)。

摘　　要：采用超声辅助乙酸法提取紫贻贝加工下脚料抗菌肽,并优化其工艺条件。结果表明,最适提取工艺条件为超声功率200 W、乙酸质量分数0.8%、液料比(V(乙酸)∶m(紫贻贝))6∶1(mL/g)、超声提取时间16min,此条件下抗菌肽得率为1.55%,提取物对金黄色葡萄球菌的抑菌圈直径为7.52mm,均显著高于传统乙酸法(P<0.05),说明此优化工艺可行,该抗菌肽具有一定的抑菌活性。The use of ultrasonic-assisted acetate extraction to improve the efficiency of the conventional acetate extraction technique with the aim of extracting antimicrobial peptides from Mytilus edulis by-product using acetate solution was investigated.Based on the single factor experiment,response surface methodology was used to optimize experimental conditions for extraction of antimicrobial peptides.The optimum extraction conditions for maximum antimicrobial peptide yield were established to be ultrasonic power of 200 W,acetate concentration of 0.8%,liquid-material ratio of 6∶1(mL/g),and ultrasonic extraction time of 16 min.Under abovementioned conditions,the yield and the zone of inhibition against Staphylococcus aureus were 1.55%and 7.52 mm,respectively.The antimicrobial peptides yield and the relative of inhibition zones were significantly higher than those of the traditional acetic acid extraction method(P<0.05).The optimized technology was feasible and the antimicrobial peptides had antibacterial activity.

关 键 词：紫贻贝 下脚料 抗菌肽 超声辅助提取 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]