绿豆抗性糊精的高效纯化技术及分子特性研究  

High Efficient Purification Technique and Molecular Characteristics of the Phaseolus radiates L.Resistant Dextrin

作　　者：李良玉 刘晚霞[3] 李朝阳 牛广财 Li Liangyu;Liu Wanxia;Li Chaoyang;Niu Guangcai(National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang;College of Forestry,Northeast Forestry University,Harbin 150040;College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang)

机构地区：[1]黑龙江八一农垦大学,国家杂粮工程技术研究中心,黑龙江大庆163319 [2]东北林业大学林学院,哈尔滨150040 [3]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《中国食品学报》2020年第10期134-141,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2017111);黑龙江省“百千万”工程科技重大专项(2019ZX06B02)。

摘　　要：以绿豆淀粉为原料,采用酸热法制备绿豆抗性糊精,利用顺序式模拟移动床色谱技术纯化绿豆抗性糊精,确定最佳技术参数为:进料量455 g/h,进水量682 g/h,循环量346 mL,在此条件下抗性糊精出口折光为24.6%,纯度99.17%,收率91.31%。采用扫描电镜、红外光谱、X射线衍射、排阻色谱-十八角度激光光散射仪-示差折光检测器联机系统、GC-MS等技术分析绿豆抗性糊精的分子特性,所得抗性糊精具有很高的纯度及优良的分子特性,为抗性糊精的开发利用奠定理论基础。In this paper,Phaseolus radiates L.starch as the raw material,the Phaseolus radiates L.resistant dextrin were prepared by acid hydrolysis method,and were purified by the sequential simulated moving chromatography(SSMB).The optimized process parameters were:feed rate 455 g/h,water consumption 682 mL/h,circulation quantity 346 mL.Under these conditions,the purified Phaseolus radiates L.resistant dextrin concentration was 24.6%,the purity was 99.17%,and the yield was 91.31%.Molecular characteristics of Phaseolus radiates L.resistant dextrin were analyzed by the SEM,FTIR,XRD,SEC-MALS and GC-MS,it had high purity and excellent molecular characteristics,it could provide theoretical principle for the development and utilization of resistant dextrin.

关 键 词：绿豆 抗性糊精 水溶性膳食纤维 制备色谱 顺序式模拟移动色谱 分子特性 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]