混合溶液中茶多酚、植酸含量的分光光度法测定及干扰消除  

Concentration Determination and Interference Elimination of the Mixed Solution of Tea Polyphenols and Phytic Acid

作　　者：张璇 李秋莹 钟克利 孙彤[1,2] 杨丽丽 韩英 励建荣 Zhang Xuan;Li Qiuying;Zhong Keli;Sun Tong;Yang Lili;Han Ying;Li Jianrong(National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,Liaoning;College of Food Science and Engineering,Food Safety Key Lab of Liaoning Province,Bohai University,Jinzhou 121013,Liaoning;College of Chemistry and Chemical Engineering,Bohai University,Jinzhou 121013,Liaoning)

机构地区：[1]生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,辽宁锦州121013 [3]渤海大学化学化工学院,辽宁锦州121013

出　　处：《中国食品学报》2020年第10期262-267,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2016YFD0400805);国家自然科学基金项目(31371858)。

摘　　要：茶多酚和植酸均可用作食品保鲜剂,复配使用效果更好。采用分光光度法同时测定混合溶液中茶多酚和植酸的含量,并消除相互之间的干扰。研究结果表明,植酸对茶多酚的显色无影响,可采用酒石酸亚铁显色法直接测得混合溶液中茶多酚的含量;而当采用三氯化铁-磺基水杨酸显色时,茶多酚和植酸均有显色,且混合溶液中茶多酚质量浓度影响植酸的显色能力。采用数学模拟法可消除茶多酚对植酸显色能力的干扰,获得植酸的标准曲线。此方法检测最佳范围为茶多酚质量浓度0~0.300 mg/mL,植酸质量浓度0~3.000 mg/mL,回收率96%~103%。方法操作简单,准确度高,可为混合组分的同时测定提供理论依据。Tea polyphenols and phytic acid both can be used as food preservative.In this paper,the mixed solutions concentrations of tea polyphenols and phytic acid were simultaneously determined by spectrophotometr,and the interference was eliminated.The results showed that phytic acid had no effect on the coloration of tea polyphenols,and the concentration of tea polyphenols in the mixed solution could be determined immediately by ferrous tartrate coloration method.Tea polyphenols and phytic acid both had color development when the polyferric chloride-sulfosalicylic acid was a chromogenic agent,and the color development ability of phytic acid were affected by the mass concentration of tea polyphenols in the mixed solution.The interference of tea polyphenols on the color development ability of phytic acid could be eliminated by the mathematical simulation method,and the standard curve of phytic acid were obtained.By this method,the optimal determination range was the tea polyphenol mass concentration of 0-0.300 mg/mL,the phytic acid mass concentration of 0-3.000 mg/mL,and the recovery rate of 96%-103%.The method is simple to operate and has high accuracy,and it can provide a theoretical basis for simultaneous determination of mixed components.

关 键 词：茶多酚 植酸 分光光度法 干扰消除 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]