8种市售核酸染料的细菌回复突变实验  

Safety of eight nucleic acid dyeson

作　　者：余文[1] 安琳 陈怡文 任秀[1] 骆海朋[1] 刘娜[1] 崔生辉[1] 李景云[1] Yu Wen;An Lin;Chen Yiwen;Ren Xiu;Luo Haipeng;Liu Na;Cui Shenghui;Li Jingyun(National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《卫生研究》2020年第6期938-943,948,共7页Journal of Hygiene Research

基　　金：国家重点研发计划(No.2018YFC1603900)。

摘　　要：目的探讨溴化乙锭(ethylene dibromide,EB)、GelGreen、GoldView、GeneGreen、SYBRGreen I、GelRed、SYBRSafe、SYBRGold这8种染料的安全性。方法使用鼠伤寒沙门菌TA97、TA98、TA100、TA102,依据美国临床实验室标准化协会(Clinical and Laboratory Standards Institute,CLSI)肉汤稀释法测定8种染料的最低抑菌浓度(minimum inhibitory concentration,MIC),依据GB 15193.4—2014进行细菌回复突变试验。结果SYBRSafe对TA97、TA100有明显的抑菌性,MIC为染料标准工作浓度的6.25倍,SYBRGreen I对4个菌的抑菌性均较为明显,TA97、TA100的MIC值为染料标准工作浓度的1.56倍,TA98、TA102的MIC为染料标准工作浓度的6.25倍。EB及其代谢产物对4个菌均有强致突变性;SYBRGold及其代谢产物对4个菌均未见致突变性;另外6种染料对TA97、TA98、TA102存在不同程度的致突变性,对TA100未见致突变性。结论除EB外,其余染料在说明书推荐的实验浓度未见致突变性;但在高浓度时,除SYBRGold外呈现不同程度的致突变性。OBJECTIVE To study the safety of eight nucleic acid dyes:EB,GelGreen,GoldView,GeneGreen,SYBRGreen I,GelRed,SYBRSafe and SYBRGold.METHODS Salmonell atyphimurium TA97,TA98,TA100 and TA102 involved in the minimum inhibitory concentration(MIC)of the 8 nucleic acid dyes by the Broth dilution,which follows Clinical and Laboratory Standards Institute.The experiment tests the reverse mutation of bacteria with the 8 nucleic acid dyes,which follows GB 15193.4-2014.RESULTS EB and its metabolites show significant mutagenicity on TA97,TA98,TA100 and TA102.SYBRGold and its metabolites do not show mutagenicity on TA97,TA98,TA100 and TA102.The other six dyes show varying mutagenicity on TA97,TA98 and TA102,meanwhile show no mutagenicity on TA100.CONCLUSION This research shows that,except EB,the other dyes show no mutagenicity under working concentration,but show varying mutagenicity under high concentration.SYBRSafe and SYBRGreenI are highly toxic,and the operators must have high precautions when making diluted solution of them.

关 键 词：核酸染料 安全性 致突变性 食品安全 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TS202[医药卫生—公共卫生与预防医学]