单糖定性定量的色谱分析方法的研究进展  

Development of Qualitative and Quantitative Chromatographic Analysis of Monosaccharides

作　　者：尹大芳 孙晓杰[1] 郭莹莹[1] 彭吉星[1] 王婧媛 李娜[1] 王联珠[1] YIN Da-fang;SUN Xiao-jie;GUO Ying-ying;PENG Ji-xing;WANG Jing-yuan;LI Na;WANG Lian-zhu(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]上海海洋大学食品学院,上海201306

出　　处：《食品工业科技》2020年第24期321-329,共9页Science and Technology of Food Industry

基　　金：现代农业产业技术体系专项资金资助(CARS-50);中国水产科学研究院黄海水产研究所基本科研业务费资助(20603022018017);中国水产科学研究院基本科研业务费资助(2020TD71)。

摘　　要：糖类为多羟基的醛酮及其缩聚物和某些衍生物的总称,是组成生物体的四大组分之一,是维持生命活动所需要能量的主要来源。糖类有单糖、寡糖和多糖3种形式,在生命体中大都以多糖的形式存在。多糖结构复杂,相对分子质量大,由10个以上的单糖组成,是重要的生物活性物质。多糖的生物活性与其单糖组成密不可分,因此,研究多糖中单糖组成的定性与定量方法具有重要的意义。单糖是一种含有多羟基的亲水性大分子,具有结构相似、亲水性、无发色基团和荧光基团的性质,其定性与定量工作极具挑战。本文以四种单糖定量色谱方法(液相色谱法、气相色谱法、高效阴离子交换色谱法、毛细管电泳法)研究现状为重点进行论述,对色谱方法中存在的问题及其优势进行分析与评述,并对单糖的色谱检测技术的发展进行展望。Carbohydrates,one of the four major components that make up an organism,are a general term for polyhydroxy aldehydes,polyhydroxy ketones,its polycondensates and derivatives,which was the main source of energy needed to maintain life activities.Carbohydrates exist in three forms:Monosaccharides,Oligosaccharides,and Polysaccharides.Most of them exist in the form of polysaccharides in living organisms.Polysaccharides have complex structures and large molecular weight,and are composed of more than 10 monosaccharides.They are important biologically active substances.The biological activity of polysaccharides is inseparable from monosaccharide composition.Therefore,it is of great significance to study the qualitative and quantitative methods of monosaccharide composition in polysaccharides.Monosaccharide is a kind of hydrophilic macromolecule containing polyhydroxy group,which is hydrophilic,no chromophoric group and fluorescent group.Its qualitative and quantitative work is very challenging.This article focuses on the research status of four kinds of quantitative methods for monosaccharide quantitative chromatography(liquid chromatography,gas chromatography,high performance anion exchange chromatography,capillary electrophoresis),analyzes the problems and advantages of chromatography methods.The development of chromatographic detection technology of monosaccharides is prospected.

关 键 词：高效液相色谱 气相色谱 毛细管电泳 高效阴离子交换色谱 单糖 检测 

分 类 号：TS201.1[轻工技术与工程—食品科学]