高效液相色谱法测定高温加工食品中丙烯酰胺  

Determination of acrylamide in high temperature processed food by high performance liquid chromatography

作　　者：战旭梅[1] 刘萍[1] 祁兴谱 胥栋豪 ZHAN Xu-Mei;LIU Ping;QI Xing-Pu;XU Dong-Hao(School of Food Science and Technology,Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院食品科技学院,泰州225300

出　　处：《食品安全质量检测学报》2020年第22期8496-8501,共6页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法测定高温加工食品中丙烯酰胺含量的分析方法。方法样品粉碎后经超纯水提取、正己烷脱脂、C18固相萃取柱萃取、流动相洗脱后得到样品净化液,经高效液相色谱仪,以乙腈-水(体积比2:98)作为流动相,流速为0.8 mL/min,测定波长205 nm,柱温20℃的色谱条件测定,外标法定量。结果在该条件下,丙烯酰胺在0.1~2.0μg/mL内具有良好的线性关系(r2=0.9987),检出限14μg/kg,定量限40μg/kg,加标回收率在91.5%~95.0%之间,1.0μg/mL丙烯酰胺标准溶液重复6次进样的相对标准偏差为(relative standard deviation,RSD)为0.16%。结论此方法可以准确、快速测定高温加工食品中的丙烯酰胺含量。Objective To establish a high performance liquid chromatography method for the determination of acrylamide in high temperature processed food.Methods The sample was extracted by ultra-pure water after crushed,degreased by n-hexane,extracted by C18 solid phase extraction column and eluted by mobile phase.The sample was purified by high performance liquid chromatography with acetonitrile water(2:98,V:V)as mobile phase.The flow rate was 0.8 mL/min,the detection wavelength was 205 nm,and the column temperature was 20℃.The external standard method was used for quantitative determination.Results Under these conditions,the linear range of acrylamide was 0.1–2.0μg/mL(r^2=0.9987),the limit of detection was 14μg/kg,the limit of quantitation was 40μg/kg,the recovery rate was 91.5%–95.0%,and the relative standard deviation(RSD)of 1.0μg/mL acrylamide standard solution after 6 injections was 0.16%(n=6).Conclusion This method can accurately and quickly determine the content of acrylamide in high temperature processed food.

关 键 词：高温加工食品 丙烯酰胺 高效液相色谱法 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]