苦竹笋总黄酮大孔树脂纯化工艺及其体外抗炎活性研究  

Purification of total flavonoids from bamboo shoots of Pleioblastus amarus with macroporous resin and its anti-inflammation activity

作　　者：晏俊玲 樊扬 秦川 欧雪 陈姝娟[1] 敖晓琳[1] YAN Junling;FAN Yang;QIN Chuan;OU Xue;CHEN Shujuan;AO Xiaolin(College of Food Science,Sichuan Agricultural University,Ya’an 625014,China)

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《食品与发酵工业》2020年第23期184-192,共9页Food and Fermentation Industries

摘　　要：采用大孔吸附树脂对苦竹笋总黄酮进行纯化,探索其最佳工艺条件,并对其富集产物进行体外抗炎活性测定。首先对树脂的筛选、吸附动力学、吸附等温线和热力学进行了研究,再进行动态吸附和解吸试验,筛选得出AB-8是最适宜纯化苦竹笋总黄酮的树脂。准二级动力学模型和Langmuir等温线模型对吸附数据进行了最佳描述,吸附过程自发、放热。确定纯化最优工艺为:上样量4.2 BV(105 mL)、上样液质量浓度1.55 mg/mL、上样流速1 BV/h、上样液pH值为7、洗脱剂为50%乙醇溶液(体积分数)、洗脱剂用量8 BV(200 mL)、洗脱流速4 BV/h,在此工艺条件下,总黄酮回收率可达到75.03%,总黄酮含量由原来的2.46%提高至15.72%。且纯化后的苦竹笋总黄酮表现出更好的NO抑制作用,纯化样品总黄酮浓度达到1.04 mg/mL抑制率可达到97.80%(NO浓度为5.60μmol/L),而同一总黄酮浓度下的粗提样品中NO浓度为13.81μmol/L,抑制率仅为73.22%。综上,该实验建立的苦竹笋总黄酮的纯化方法有效可行。The aim of this work is to develop an efficient and economical method for the enrichment of total flavonoids from the shoots of P.amarus,and to determine its anti-inflammatory activity in vitro.Before the dynamic adsorption and desorption tests,the resin screening,adsorption kinetics,adsorption isotherm and thermodynamics were studied successively.The screening showed that AB-8 resin is the most suitable resin for purifying the total flavonoids of the shoots of P.amarus.The static experimental data correlated well with the pseudo-second-order kinetics model and Langmuir isotherm model.The adsorption process is spontaneous and exothermic.The optimum enrichment conditions were as follows:for adsorption the total flavonoids concentration,flow rate and volume of sample were 1.55 mg/mL,1 BV/h and 4.2 BV.Respectively,and for desorption the flavonoids-loaded AB-8 resin column was desorbed by 8 BV of 50%ethanol at a rate of 4 BV/h,the recovery yield of total flavonoids was 75.03%,total flavonoids content increased from 2.46%to 15.72%.The total flavonoids of the purified could better inhibit the production of NO.When the total flavonoids concentration reached 1.04 mg/mL,the inhibition rate reached 97.80%(the concentration of NO was 5.60 mol/L),while the concentration of NO in the crude extracts was 13.81 mol/L,inhibition rate was only 73.22%.In summary,the purification method of total flavonoids of the shoots of P.amarus established in this experiment is effective and feasible.

关 键 词：苦竹笋 总黄酮 大孔吸附树脂 吸附动力学 吸附热力学 抗炎活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]