Ba^2+对南美白对虾酚氧化酶活性和结构的影响  

Effects of Barium Ions on the Activity and Structure of Phenoloxidase

作　　者：佟抒洋 张书琪 郭雨晴 张俊健 张弛 吕艳芳 TONG Shuyang;ZHANG Shuqi;GUO Yuqing;ZHANG Junjian;ZHANG Chi;LU Yanfang(College of Food Science and Technology,Bohai University,Jinzhou 121013)

机构地区：[1]渤海大学食品科学与工程学院,锦州121013

出　　处：《食品工业》2020年第11期181-185,共5页The Food Industry

基　　金：大学生创新创业训练项目(201910167230);渤海大学博士启动项目(0519bs010)。

摘　　要：为研究金属离子对南美白对虾酚氧化酶(PO)活性和结构的影响,以南美白对虾头胸部分离纯化出的PO酶为原料,研究了不同浓度钡离子(Ba^2+)对PO酶活性和结构的影响。通过紫外光谱扫描、荧光扫描,研究了Ba^2+对酚氧化酶三级结构的影响;通过红外光谱,研究了酚氧化酶与Ba^2+结合后,其二级结构类型的变化。结果显示,随着Ba^2+浓度的不断增加,酚氧化酶的紫外光谱和荧光光谱的强度均呈现先增大后减小的趋势,但并未发生红移或蓝移现象,说明酚氧化酶与Ba^2+结合后,三级结构变化很小;根据红外光谱扫描结果和拟合结果发现,酚氧化酶与Ba^2+结合后,二级结构类型发生了改变。The purified phenol oxidase(PO) from Penaeus vannamei was treated by different concentrations of Ba^2+ to investigate the effect of metal ions on the activity and structure of PO. UV spectroscopy, fluorescence scanning and infrared spectroscopy were applied to observe the structural changes of PO. The results showed that with the concentration of Ba^2+ increasing, the intensity of the UV and the fluorescence spectrum decreased after an initial increasing, while no red shift or blue shift occurred, indicating the binding of PO and Ba^2+ lead to minor changes in its tertiary structure. The infrared spectrum scanning and fitting results indicated that the secondary structure of PO changed after the Ba^2+ binding.

关 键 词：南美白对虾 酚氧化酶 酶活 三级结构 二级结构 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]