椴树蜜纯度鉴定及其抗氧化活性研究  

Purity Identification of Linden Honeys and Their Antioxidant Activity

作　　者：张孟历 张宏武[2] 贾红梅[2] 于猛[2] 王宝龙 邹忠梅[2] ZHANG Meng-li;ZHANG Hong-wu;JIA Hong-mei;YU Meng;WANG Bao-long;ZOU Zhong-mei(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;The Institute of Medicinal Plant Development,Beijing 100193,China;China Association for Quality Promotion Ecological Apiculture Committee,Beijing 100031,China)

机构地区：[1]天津中医药大学,天津301617 [2]中国医学科学院药用植物研究所,北京100193 [3]中国质量万里行促进会生态蜂业专业委员会,北京100031

出　　处：《食品研究与开发》2021年第1期55-59,共5页Food Research and Development

摘　　要：测定东北地区收集的16批椴树蜜中椴树花粉占总花粉比例;并采用福林酚法测定各批次椴树蜜中的总酚含量;采用DPPH自由基清除法、ABTS+自由基清除法与铁离子还原能力(ferric reducing antioxidant power,FRAP)法测定各批次椴树蜜的总抗氧化能力。结果表明:16批椴树蜜中椴树花粉占总花粉含量分别在7.14%~76.73%之间,椴树花粉含量可用于椴树蜜纯度鉴定,其中7个批次的椴树蜜判定为椴树单花蜜,9个批次椴树蜜为椴树杂花蜜;椴树单花蜜的总酚含量以没食子酸计在174.64μg/g~222.82μg/g,杂花蜜为223.73μg/g~329.18μg/g;DPPH法测得椴树单花蜜的IC50值为(46.61±10.91)g/L~(107.76±34.17)g/L,杂花蜜IC50值为(24.72±6.89)g/L~(76.75±8.09)g/L;测得椴树单花蜜ABTS+自由基清除能力以Trolox计为(0.14±0.07)mmol/kg~(0.43±0.10)mmol/kg,椴树杂花蜜ABTS+自由基清除能力为(0.25±0.01)mmol/kg~(0.56±0.04)mmol/kg;测得椴树单花蜜铁离子还原能力以FeSO4计为(1.96±0.08)mmol/kg~(2.82±0.08)mmol/kg,椴树杂花蜜铁离子还原能力以FeSO4计为(2.08±0.07)mmol/kg~(3.81±0.11)mmol/kg。结果显示,椴树蜜有一定的抗氧化活性,抗氧化活性与总酚含量呈正相关,但与椴树蜜纯度相关性不强。The linden pollen to total pollen ratio in 16 batches of linden honey collected in Northeast China was determined.The total phenolic content in each batch of linden honey was determined by Folin-Ciocalteu method.The total antioxidant capacity of each batch of linden honey was determined by DPPH method,ABTS+method and FRAP method.The results showed that the content of linden pollen in linden honey ranged from 7.14%to 76.73%,which could be used for purity identification of linden honey.Among them,7 batches of linden honey could be judged as linden monofloral honey,and 9 batches were linden polyfloral honey.The total phenolic content of linden monofloral honey was between 174.64μg/g to 222.82μg/g in gallic acid,and linden polyfloral honey was between 223.73μg/g to 329.18μg/g in gallic acid.The IC50 value of linden monofloral honey was between(46.61±10.91)g/L to(107.76±34.17)g/L,and the IC50 value of linden polyfloral honey was between(24.72±6.89)g/L to(76.75±8.09)g/L.The ABTS+free radical clearance ability of linden monofloral honey was between(0.14±0.07)mmol/kg to(0.43±0.10)mmol/kg in Trolox,the ABTS+free radical clearance ability of linden polyfloral honey was between(0.25±0.01)mmol/kg to(0.56±0.04)mmol/kg in Trolox.The ferric ion reduction ability of linden monofloral honey was between(1.96±0.08)mmol/kg to(2.82±0.08)mmol/kg in FeSO4,and the ferric ion reducing ability of linden polyfloral honey was between 2.08±0.07 to 3.81±0.11 mmol/kg in FeSO4.The results showed that all batches of linden honeys showed antioxidant activity,and their antioxidant activity strongly correlated with the total phenol content,but not with the purity.

关 键 词：蜂蜜 椴树蜜 单花蜜 抗氧化活性 总酚含量 

分 类 号：S896.1[农业科学—特种经济动物饲养]