固相微萃取法对不同月季品种花朵香气成分的GC-MS分析  

Aroma profile identification of different rose cultivars by gas chromatography-mass spectrometry coupled to headspace solid phase microextraction

作　　者：孙海楠 嵇震宇 汪有良[1,2] Sun Hainan;Ji Zhenyu;Wang Youliang(JiangsuAcademy of Forestry, Nanjing 211153, China;Rose Germplasm Resources Nursery, The Jiangsu Provincial Platform for Conservation and Utilization of AgriculturalGermplasm, Nanjing 211153, China;Jurong Dongjin Forest Farm, Jurong 212400, China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]江苏省农业种质资源保护与利用平台月季种质资源圃,江苏南京211153 [3]句容市东进林场,江苏句容212400

出　　处：《江苏林业科技》2020年第6期1-6,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省林业科技创新与推广项目“美丽宜居乡村景观绿化模式与技术集成示范”(LYKJ[2018]01);江苏省林业科学研究院青年基金项目“芳香型月季花朵香气成分鉴定及分析”(JAF-2016-05)。

摘　　要：采用顶空-固相微萃取(HS-SPME)结合气质联用(GC-MS)对5个芳香月季品种(‘香粉蝶’‘杰·乔伊’‘粉和平’‘紫红香’和‘月月粉’)的花朵香气成分进行了鉴定与分析。结果表明SPME对上述月季花朵香气成分的有效提取率在97.54%—98.57%之间,共有45种香气成分被检出。其中,烯烃类物质种类最多,为12种,主要由单萜与倍半萜组成,其次为醇类物质,数量为10种,酯类、醛类分别为9种与6种,酸类、酮类、酚类等其他物质含量较少;从分子结构类型看,45种香气成分中萜类物质共23种,苯环类化合物共12种,脂肪酸及衍生物5种,其他类型化合物5种。供试月季花朵香气中重要的成分有乙酸叶醇酯、3,5-二甲氧基甲苯、1,3,5-三甲氧基苯、樟脑、香叶醇等,其中乙酸叶醇酯在所有供试品种中都为主要成分,3,5-二甲氧基甲苯为‘香粉蝶’中特有的主要成分,樟脑为‘月月粉’中特有的主要成分。The aroma profile of 5 rose cultivars was investigated by headspace solid-phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS).The extraction efficiency between 97.54%—98.57%was obtained.And tatally 45 aroma-active compounds were identified in all tested cultivars,including 12 olefins,10 alcohols,9 esters,6 aldehydes,2 ketones,2 phenols,2 acids,1 ether,and 1 alkane.In molecular structure level,they could be categorized into 23 terpenes and oxygenated derivatives,12 aromatic compounds,5 fatty acids and their derivatives,and 5 other compounds.Among them,cis-3-hexenyl acetate,3,5-dimethoxytoluene,1,3,5-trimethoxybenzene,camphor and geraniol were major components in rose aroma profile.Especially,cis-3-hexenylacetate could be responsible for the aroma of all tested cultivars.

关 键 词：月季 香气成分 气相色谱-质谱联用 顶空-固相微萃取 

分 类 号：Q946.4[生物学—植物学] Q946.81