枸杞多糖的羧甲基化修饰及抗氧化性能  

Carboxymethylation Modification and Antioxidant Activities of Lycium barbarum Polysaccharide

作　　者：许春平 姚延超 白家峰[2] 徐石磊[2] 贾学伟 刘绍华[2] XU Chunping;YAO Yanchao;BAI Jiafeng;XU Shilei;JIA Xuewei;LIU Shaohua(College of Food&Biology Engineering,Zhengzhou University of Light Industry,Zhengzhou 450000,China;Technical Center,China Tobacco Guangxi Industrial Co.,Ltd.,Nanning 530001,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,河南郑州450000 [2]广西中烟工业有限责任公司技术中心,广西南宁530001

出　　处：《河南科技大学学报（自然科学版）》2021年第3期85-89,M0007,M0008,共7页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家自然科学基金联合项目(U1604176);广西中烟合作项目(20171011)。

摘　　要：为了研究枸杞多糖及其羧甲基化衍生物的结构和抗氧化性能,使用微波辅助酶解法提取枸杞多糖并进行羧甲基化修饰。采用高效液相色谱仪(HPLC)、傅里叶变换红外光谱仪(FTIR)和尺寸排阻色谱-多角度激光光散射-示差折光联用仪(SEC-MALLS-RI),对枸杞多糖进行了结构表征,对枸杞多糖及其羧甲基化衍生物的抗氧化性能进行了测定。研究结果表明:枸杞多糖的提取率为4.32%(质量分数),羧甲基化枸杞多糖取代度为0.43。枸杞多糖主要由甘露糖(40.05%,摩尔分数,下同)、葡萄糖醛酸(18.98%)、阿拉伯糖(18.34%)、鼠李糖(15.73%)和葡萄糖(6.90%)组成。枸杞多糖的数均相对分子质量为3.561×10^4,质均相对分子质量为5.059×10^4,多分散系数为1.421,均方根旋转半径为73.0 nm。在相同质量浓度下,羧甲基化枸杞多糖的抗氧化性能高于枸杞多糖。In order to study the structure and antioxidant activities of Lycium barbarum(L.barbarum)polysaccharide and its carboxymethylation derivative,the L.barbarum polysaccharide was extracted by microwave-assisted enzymatic hydrolysis method and was modified by carboxymethylation.The structure of L.barbarum polysaccharide was characterized by high performance liquid chromatograph(HPLC),Fourier transform infrared spectrometer(FT-IR)and size exclusion chromatography-multi-angle laser light scattering-refractive index(SEC-MALLS-RI).The antioxidant activities of L.barbarum polysaccharide and its carboxymethylation derivative were determined.The results show that the extraction rate of L.barbarum polysaccharide is 4.32%(mass fraction),and the substitution degree of carboxymethylated L.barbarum polysaccharide is 0.43.The L.barbarum polysaccharide is mainly composed of mannose(40.05%,mole fraction,the same below),glucuronic acid(18.98%),arabinose(18.34%),rhamnose(15.73%)and glucose(6.90%).The number-average relative molecular weight of L.barbarum polysaccharide is 3.561×10^4,the mass-average relative molecular weight is 5.059×10^4,the polydispersity coefficient is 1.421,and the root mean square rotation radius is 73.0 nm.At the same mass concentration,the antioxidant activities of carboxymethylated L.barbarum polysaccharide are higher than that of L.barbarum polysaccharide.

关 键 词：枸杞多糖 提取 羧甲基化 抗氧化性能 

分 类 号：TS24[轻工技术与工程—制糖工程]