曲酸高产菌的复合诱变选育  

Compound Mutagenesis of Strain with High Yield of Kojic Acid

作　　者：高紫君 毕付提 蒋水星 刘博雅 史亚楠 王德培[1,3] GAO Zi-jun;BI Fu-ti;JIANG Shui-xing;LIU Bo-ya;SHI Ya-nan;WANG De-pei(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;RZBC GROUP CO.,LTD.,Rizhao 276800,Shandong,China;Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]山东日照金禾博源生化有限公司,山东日照276800 [3]工业发酵微生物教育部重点实验室,天津300457

出　　处：《食品研究与开发》2021年第2期186-190,共5页Food Research and Development

基　　金：山东省泰山产业领军人才培育计划(2016GRC3201);国家自然科学基金青年科学基金项目(31902193)。

摘　　要：以米曲霉(Aspergillus oryzae)3.042为出发菌株,其孢子在无菌水中孵育4 h后,首先经两次紫外照射诱变,筛选获得产曲酸能力提高的突变株UV15。随后将UV15继续进行超声波和微波照射的复合诱变筛选,获得高产菌株M22,将其传代10次后,最终确定M22是一株生长性能和产酸能力均稳定的曲酸高产菌株。该菌株在接种量为104个/mL、30℃、摇床250 r/min、发酵时间6.5 d的条件下,产酸量由原来的11.55 g/L提高到34.28 g/L,比出发菌株提高了197%。In order to increase the yield of kojic acid,the spores of Aspergillus oryzae(A.oryzae)3.042 swelled in steriled water for 4 hours and were mutated twice by UV ultraviolet irradiation,a high-yield strain UV15 was obtained.Then the combined mutagenesis screening of ultrasonic and microwave irradiation was continued for UV15,and a high-yield strain M22 was obtained.After 10 generations screening,M22 was stable in growth performance and kojic acid production capacity.The acid yield of A.oryzae M22 increased from 11.55 g/L to 34.28 g/L,which was 197%higher than that of the original strain at inoculation 104 spores/mL,30℃,250 r/min,and fermentation 6.5 days.

关 键 词：米曲霉 曲酸 紫外诱变 超声微波诱变 发酵 

分 类 号：TQ921[轻工技术与工程—发酵工程]