体外亚硝化模拟体系优选阻断NPYR生成的复合香辛料精油配比  

Optimization of the Proportion of Essential Oil of Compound Spices Blocking NPYR Production in Vitro Nitrosation Simulation System

作　　者：陈文静 任小青 杨华 马俪珍 CHEN Wenjing;REN Xiaoqing;YANG Hua;MA Lizhen(College of Food Science and Bioengineering,Tianjin Agricultural University,Tianjin 300384,China;College of Animal Science and Animal Medicine,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院食品科学与生物工程学院,天津300384 [2]天津农学院动物科学与动物医学学院,天津300384

出　　处：《食品工业科技》2021年第3期6-12,共7页Science and Technology of Food Industry

基　　金：“十三五”国家重点研发计划重点专项(2018YFD0401200)。

摘　　要：为了有效抑制N-亚硝基吡咯烷(N-nitrosopyrrolidine,NPYR)的形成,在体外模拟亚硝化反应体系中,先通过单因素实验研究市售常见的8种超临界萃取香辛料精油(Spices essential oil,SEO),包括花椒精油、八角精油、青花椒精油、丁香精油、迷迭香精油、黑胡椒精油、当归风味姜油、砂仁精油,对NPYR的抑制作用,再利用响应面中心组合试验设计,筛选复合香辛料精油(Compound spices essential oil,CSEO)的最佳配比。单因素实验结果表明,除砂仁精油外,其他7种SEO对NPYR的形成均有抑制作用,抑制率大小与其添加浓度有一定关系。其中迷迭香精油(15、20.0 mL/L)对NPYR的抑制作用最强(80%以上);其次花椒精油(5.0、10.0 mL/L)、青花椒精油(0.5 mL/L)和丁香精油(15.0 mL/L)对NPYR的抑制率可以达到68%以上;当归风味姜油(10.0 mL/L)和八角精油(15.0 mL/L)的抑制效果略低(50%以上),而黑胡椒精油对NPYR的抑制率则相对较弱(低于50%)。响应面中心组合设计试验得到对NPYR抑制效果最优的CSEO配比为:441.0μL/L花椒精油、337.0μL/L八角精油、17.0μL/L青花椒精油、14.0μL/L丁香精油和137.0μL/L迷迭香精油,由模型优选得出对NPYR抑制率的预测值为69.84%,验证试验得出的抑制率为68.40%,CSEO对N-二甲基亚硝胺(N-nitrosodimethylamine,NDMA)的抑制率为66.69%。In order to effectively inhibit the formation of N-nitrosopyrrolidine(NPYR),in the simulated nitrosation reaction system in vitro,a single factor test was firstly conducted to study the eight common supercritical extraction spices essential oils(SEO),including pepper essential oil,star anise essential oil,green pepper oil,clove essential oil,rosemary essential oil,black pepper essential oil,angelica flavored ginger oil,amomum essential oil,inhibitory effect on NPYR,and response surface center combination test was used to screen the best ratio of compound spices essential oil(CSEO).The single-factor test results showed that,except for amomum villosum essential oil,the other seven SEOs all had an inhibitory effect on the formation of NPYR,and the inhibition rate had a certain relationship with its added concentration.Among them,rosemary essential oil(15、20.0 mL/L)had the strongest inhibitory effect on NPYR(more than 80%),followed by pepper essential oil(5.0,10.0 mL/L),green pepper oil(0.5 mL/L)and clove essential oil(15.0 mL/L)against NPYR.The inhibition rate could reach more than 68%,the inhibitory effect of angelica flavor ginger oil(10.0 mL/L)and star anise oil(15.0 mL/L)was slightly lower(more than 50%),while the inhibitory rate of black pepper essential oil on NPYR was relatively weak(less than 50%).The CSEO composition with the best NPYR inhibition effect was obtained from the response surface center design test:441.0μL/L peppercorn essential oil,337.0μL/L star anise essential oil,17.0μL/L green pepper oil,14.0μL/L clove essential oil,and 137.0μL/L rosemary essential oil,the predicted value of the NPYR inhibition rate was 69.84%from the model optimization,the inhibition rate was 68.40%from the verification test,and the inhibition rate of CSEO against N-nitrosodimethylamine(NDMA)was 66.69%.

关 键 词：体外模拟体系 香辛料精油 N-亚硝基吡咯烷(NPYR) 响应面中心组合设计 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]