酶法催化紫苏油和MCT合成中长链甘油三酯研究  

Enzymatic Synthesis of Medium-and Long-chain Triacylglycerols Based on Perilla Oil and MCTs

作　　者：黄昭先 王满意 孙承国 王风艳 王翔宇 江连洲[4,5] HUANG Zhaoxian;WANG Manyi;SUN Chengguo;WANG Fengyan;WANG Xiangyu;JIANG Lianzhou(COFCO Nutrition and Health Research Institute,Beijing 102209,China;Beijing Key Laboratory of Nutrition&Health and Food Safety,Beijing 102209,China;R&D Center,COFCO-Oils,Tianjin 300452,China;College of Food Science,Northeast Agricultural University,Harbin 150030,China;Linyi Yuwang Plant Protein Co.,Ltd.,Dezhou 253000,China)

机构地区：[1]中粮营养健康研究院,北京102209 [2]营养健康与食品安全北京市重点实验室,北京102209 [3]中粮油脂专业化公司研发中心,天津300452 [4]东北农业大学食品学院,哈尔滨150030 [5]临邑禹王植物蛋白有限公司,德州253000

出　　处：《农业机械学报》2021年第1期360-366,359,共8页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家重点研发计划项目(2016YFD0401405);山东省重点研发计划项目(2018YYSP026);山东省德州市大禹英才项目;山东省泰山产业领军人才工程高校生态农业创新类项目(LJNY201607)

摘　　要：通过Lipozyme RM IM催化紫苏油与中链甘油三酯(MCT)进行酯交换合成富含α-亚麻酸的中长链甘油三酯(MLCT),研究了酶添加量、底物中MCT浓度、反应温度和反应时间对MLCT得率的影响。40%MCT和60%紫苏油在6%的Lipozyme RM IM催化作用下,于60℃进行4 h的酯交换反应,结果表明,在反应平衡后,MLCT得率超过70%。采用两步法除臭工艺纯化酯交换产物,产品中的甘油三酯纯度达到97%,游离脂肪酸含量显著降低,反式脂肪酸质量分数可以控制在1%以内。此外,在纯化产物中存在超过40%的α-亚麻酸,超过85%的长链脂肪酸在甘油三酯中的sn-2位置。The medium-and long-chain triacylglycerols(MLCTs)rich inα-linolenic acid could be used as an anti-obesity,functional material in food industry.MLCTs could overcome the shortcomings of the LCTs and MCTs,and provide the human body with essential fatty acids and faster energy supply.Enzymatic interesterification of perilla oil and MCTs was performed to prepare the MLCTs rich inα-linolenic acid,the reaction were catalysed by Lipozyme RM IM.The effects of enzyme loading,ratio of MCTs to perilla oil,reaction time and reaction temperature on the yield of MLCT were studied.The results showed that the reaction achieved more than 70%yield of MLCTs in triacylglycerols under the conditions of 6%Lipozyme RM IM,40%MCT and 60%perilla oil at 60℃for 4 h.Deodorization was a traditional deacidification method,in consideration of processing costs and equipment reconstruction costs,the two-stages deodorization was used to update into a two-stages deodorization to better remove the undesired by-products after interesterification reaction,providing a method for the physical deacidification of polyunsaturated oils in practice.The free fatty acids of the interesterified products were removed significantly,the triacylglycerols were more than 97%in the refined products,and the trans fatty acids could be limited within 1%.In the purified products,more than 85%of the long-chain fatty acids were occupying the sn-2 position in triacylglycerols,and the totalα-linolenic were more than 40%.

关 键 词：中长链甘油三酯 Α-亚麻酸 酶促酯交换 两步法除臭 

分 类 号：TS201.2[轻工技术与工程—食品科学]