牛α-乳白蛋白-油酸复合物对肿瘤细胞能量代谢的影响  

Effect of Bovineα-Lactalbumin-oleic Acid Complex on Energy Metabolism of Tumor Cells

作　　者：杨志轩 方冰[1] 任发政[1] 冷小京[1] Yang Zhixuan;Fang Bing;Ren Fazheng;Leng Xiaojing(Key Laboratory of Functional Dairy,Ministry of Education,Department of Nutrition and Health,China Agricultural University,Beijing 100083)

机构地区：[1]中国农业大学营养与健康系教育部-北京市共建功能乳品重点实验室,北京100083

出　　处：《中国食品学报》2021年第1期38-43,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市科委项目(D171100008017003)。

摘　　要：本研究以人肝癌细胞(HepG2)为对象,分为α-LA-OA处理组、牛-α乳白蛋白(α-LA)处理组和溶剂对照组,以细胞的糖酵解和线粒体有氧呼吸2个过程中胞外酸化率和细胞耗氧率的变化水平为衡量指标,评估α-LA-OA对肿瘤细胞能量代谢的影响。结果表明,α-LA-OA能够显著性降低人肝癌细胞的糖酵解能力以及线粒体有氧呼吸能力,并降低ATP的产生量(P<0.05)和基础呼吸(P<0.05),而α-LA处理组和溶剂对照组均无上述作用(P>0.05)。结论:α-LA-OA能够降低肿瘤细胞的能量代谢水平。本研究为开发靶向能量代谢的抗肿瘤药物提供理论依据。In this study,human hepatoma cells(HepG2)were treated withα-LA-OA,bovine-α-lactalbumin(α-LA)and the control solution.The effects ofα-LA-OA on glycolysis and mitochondrial aerobic respiration were evaluated by the Seahorse Flux.These results indicated thatα-LA-OA significantly reduced the glycolysis ability and mitochondrial aerobic respiration ability of HepG2,as well as the ATP levels(P<0.05),whereasα-LA and the control solution did not exhibit the above effects(P>0.05).This study laid a theoretical basis for the development of tumor-targeted drugs.

关 键 词：牛α-乳白蛋白-油酸 肿瘤细胞 能量代谢 糖酵解 有氧呼吸 

分 类 号：R730.5[医药卫生—肿瘤]