章鱼墨囊黑色素的提取及理化性质研究  

Studies on the Extraction and Physicochemical Properties of Melanin from Octopus Ink Sac

作　　者：李娇 王向红[1] 周畅 李希羽 智童心 桑亚新[1] Li Jiao;Wang Xianghong;Zhou Chang;Li Xiyu;Zhi Tongxin;Sang Yaxin(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,Hebei)

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《中国食品学报》2021年第1期166-171,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2019YFD0902003-03);河北省重点研发计划项目(18273201D)。

摘　　要：为实现章鱼废弃物的高值化利用,以章鱼墨囊为原料,对比高速离心法和酶解法2种章鱼墨黑色素的提取方法,并对其超微结构、紫外-可见光谱及理化性质进行研究。结果表明,高速离心法提取的黑色素在紫外区波长230 nm和326 nm处有2个特征吸收峰,平均粒径为123 nm,结构完好。理化性质分析表明其不溶于大部分有机、无机试剂,在酸性溶液中沉淀,在碱性缓冲液中微溶,在NaOH溶液中溶解性良好;在自然光照条件下该黑色素稳定性较好,在紫外光下不耐受。在长时间高温加热条件下其稳定性较差,并且不同pH值条件下,随着溶液碱性的增强,章鱼墨黑色素的稳定性逐渐升高。本研究为章鱼墨囊黑色素的综合利用提供数据支持。In order to realize the high-value utilization of octopus waste,the octopus ink sac was used as the raw material.The study compared high-speed centrifugal method and enzyme method to extract octopus ink melanin,and explore its ultrastructure,ultraviolet-visible spectrum,physical and chemical properties.The results showed that the melanin extracted by high-speed centrifugation has two characteristic absorption peaks at the wavelength of 230 nm and 326 nm in the ultraviolet region.The average particle size is 123 nm,and the structure is intact.Analysis of physicochemical properties of octopus melanin indicates it was insoluble in most organic and inorganic reagents,would precipitate in acidic solution,was slightly soluble in alkaline buffer,and had good solubility in NaOH solution;under natural light conditions,the octopus melanin had good stability,but it was intolerant in the ultraviolet light.Under long time high temperature heating condition,the melanin was instable.Under different pH value conditions,the stability of octopus melanin increased with the increase of solution alkalinity.This study provides data support for the comprehensive utilization of melanin in octopus ink sacs.

关 键 词：章鱼墨囊 结构 黑色素 理化性质 

分 类 号：TS264.4[轻工技术与工程—发酵工程]