Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective  

作　　者：Menekse Ermis Ezgi Antmen Vasif Hasirci 

机构地区：[1]BIOMATEN,Middle East Technical University(METU)Center of Excellence in Biomaterials and Tissue Engineering,Ankara,Turkey [2]METU,Department of Biomedical Engineering,Ankara,Turkey [3]METU,Department of Biotechnology,Ankara,Turkey [4]METU,Department of Biological Sciences,Ankara,Turkey

出　　处：《Bioactive Materials》2018年第3期355-369,共15页生物活性材料（英文）

摘　　要：Cell-substrate interactions play a crucial role in the design of better biomaterials and integration ofimplants with the tissues. Adhesion is the binding process of the cells to the substrate through interactionsbetween the surface molecules of the cell membrane and the substrate. There are severalfactors that affect cell adhesion including substrate surface chemistry, topography, and stiffness. Thesefactors physically and chemically guide and influence the adhesion strength, spreading, shape and fate ofthe cell. Recently, technological advances enabled us to precisely engineer the geometry and chemistry ofsubstrate surfaces enabling the control of the interaction cells with the substrate. Some of the mostcommonly used surface engineering methods for eliciting the desired cellular responses on biomaterialsare photolithography, electron beam lithography, microcontact printing, and microfluidics. Thesemethods allow production of nano- and micron level substrate features that can control cell adhesion,migration, differentiation, shape of the cells and the nuclei as well as measurement of the forces involvedin such activities. This review aims to summarize the current techniques and associate these techniqueswith cellular responses in order to emphasize the effect of chemistry, dimensions, density and design ofsurface patterns on cell-substrate interactions. We conclude with future projections in the field of cellsubstrateinteractions in the hope of providing an outlook for the future studies.

关 键 词：MICROFABRICATION MICROPATTERN Cell-material interaction DIFFERENTIATION Cell adhesion 

分 类 号：TG1[金属学及工艺—金属学]