超高效液相色谱-四极杆-飞行时间高分辨质谱法快速筛查柠檬果实中的生物活性成分  

Ultra-performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry method for rapid screening of bioactive compounds in lemon fruits

作　　者：庞雯辉 赵希娟 陈西[3] 张耀海 王成秋[1] 赵其阳[1] 焦必宁[1] PANG Wenhui;ZHAO Xijuan;CHEN Xi;ZHANG Yaohai;WANG Chengqiu;ZHAO Qiyang;JIAO Bining(Quality Supervision and Testing Center for Citrus and Seedling,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Citrus Quality and Safety,Citrus Research Institute,Chinese Academy of Agricultural Sciences,Southwest University,Chongqing 400712,China;College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;SCIEX Analytical Instrument Trading Co.Ltd.,Shanghai 200335,China)

机构地区：[1]中国农业科学院,西南大学柑桔研究所,农业农村部柑桔产品质量安全风险评估实验室(重庆),农业农村部柑桔及苗木质量监督检验测试中心,重庆400712 [2]西南大学园艺园林学院,重庆400715 [3]上海爱博才思分析仪器贸易有限公司,上海200335

出　　处：《食品与发酵工业》2021年第4期222-230,I0001-I0003,共12页Food and Fermentation Industries

基　　金：国家现代农业(柑桔)产业技术体系建设专项(CARS-26);国家农产品质量安全风险评估重大专项(GJFP2019043);国家重点研发专项(2016YFF0201105)。

摘　　要：基于前期实验室构建的生物活性成分质谱数据库,该文建立了同时筛查并准确鉴定类黄酮、香豆素、酚酸、生物碱及类柠檬苦素多类型生物活性成分的超高效液相色谱-四极杆-飞行时间高分辨质谱方法,可快速、准确筛查6种柠檬不同部位(果皮、果肉与果汁)的生物活性成分,结果表明,在6种柠檬样品中共鉴定出40种类黄酮、27种香豆素、9种酚酸、7种生物碱和3种类柠檬苦素。扁红柠檬、北京柠檬、柯尔提粗柠檬、尤力克柠檬、里斯本柠檬和美国粗柠檬分别筛查鉴定出72、71、70、66、64和63种生物活性成分。不同部位鉴定的生物活性成分数量呈以下规律:果皮≥果肉≥果汁。该方法经过实际样品验证,并可广泛适用于柑橘类水果中生物活性成分筛查分析鉴定。Base on the bioactive compounds mass spectrometry database established by our laboratory,this study established an ultra performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry(UPLC-Q-TOF-HRMS)method for simultaneous screening and accurate identification of bioactive compounds,such as flavonoids,coumarins,phenolic acids,alkaloids and limonoids,which was further applied for rapid and accurate screening of bioactive compounds of six different parts of lemons(peel,pulp and juice).The result showed that 40 flavonoids,27 coumarins,9 phenolic acids,7 alkaloids and 3 limonoids were determined in the six kinds of lemon samples.Specifically,72,71,70,66,64 and 63 kinds of bioactive compounds were identified in the screening of Bianhongningmeng,Beijingningmeng,Keerticuningmeng,Youlikeningmeng,and Lisibenningmeng,and Meiguocuningmeng,respectively.The number of bioactive compounds in different parts showed the following order:peel≥pulp≥juice.Through actual sample analysis,this method can be used in the identification of bioactive compounds in lemon and other citrus fruits.

关 键 词：超高效液相色谱-四极杆-飞行时间高分辨质谱 质谱数据库 信息依赖扫描 生物活性成分 柠檬 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]