鳕鱼皮明胶肽硒复合物的制备及结构表征  

Preparation and Structural Characterization of Peptide-Selenium Complex from Hoki(Macruronus novaezelandiae)Skin Gelatin

作　　者：吴佳南 孙娜[1,2] 林松毅 吴汶飞 WU Jianan;SUN Na;LIN Songyi;WU Wenfei(School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China;National Engineering Research Center of Seafood,Dalian 116034,China)

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]国家海洋食品工程技术研究中心,辽宁大连116034

出　　处：《食品科学》2021年第4期87-93,共7页Food Science

基　　金：辽宁省博士科研启动基金计划项目(2019-BS-017)。

摘　　要：以新西兰鳕鱼皮为原料,提取鳕鱼皮明胶并酶解获得鳕鱼皮明胶肽,以亚硒酸钠为硒源,制备并筛选出硒结合量较高的肽硒复合物,对其进行结构表征。结果显示,鳕鱼皮明胶胃蛋白酶酶解物的硒结合含量最高(13.61μg/mg),因此将其用于制备鳕鱼皮明胶肽硒复合物;鳕鱼皮明胶肽与硒结合后,紫外光谱吸收峰强度增大且红移,荧光光谱吸收峰由波长311 nm迁移到355 nm且荧光强度减弱,表明鳕鱼皮明胶肽与硒结合产生新的物质;基于粒径分布、原子力显微镜、扫描电子显微镜、X射线衍射分析,鳕鱼皮明胶肽硒复合物呈现为纳米晶体结构,主要是由于鳕鱼皮明胶肽上的羧基和氨基与硒发生了结合反应,使得β-折叠结构转变为α-螺旋结构和β-转角结构,从而使得结构更加紧凑有序。本研究制备了一种新型的肽硒复合物,为实现鱼皮高值化利用提供了新途径。In this study,gelatin was extracted from the skin of New Zealand hoki(Macruronus novaezelandiae),and was further hydrolyzed enzymatically to produce peptides.Peptide-selenium complexes were prepared by using sodium selenite as a selenium source and the one with high selenium-binding capacity was selected and structurally characterized.The results indicated that the pepsin hydrolysate had the highest calcium binding capacity(13.61μg/mg)and was thus used to peptide-selenium complexes.After selenium binding,the UV absorption intensity of the peptides increased,accompanied by a red shift in the maximum wavelength,and the fluorescence absorption peak shifted from 311 to 355 nm together with a weakening of the fluorescence intensity.These phenomena indicated the production of a new complex from selenium binding to the peptides.Based on particle size distribution measurement,atomic force microscopy(AFM),scanning electron microscopy(SEM),and X-ray diffraction analysis,the peptide-selenium complex had a nanocrystalline structure.The binding reaction of carboxyl and amino groups in the peptides with selenium transformed the secondary protein fromβ-sheet toα-helix and β-turn,thereby making the structure more compact and orderly.This study provides a new approach for highvalue utilization of fish skin.

关 键 词：鳕鱼皮 明胶肽 硒 肽硒复合物 结构表征 

分 类 号：TS209[轻工技术与工程—食品科学]