Graphene quantum dots: From efficient preparation to safe renal excretion  

作　　者：Caroline Hadad JoséMiguel González-Domínguez Silvia Armelloni Deborah Mattinzoli Masami Ikehata Akcan Istif Adrian Ostric Francesco Cellesi Carlo Maria Alfieri Piergiorgio Messa Belén Ballesteros Tatiana Da Ros 

机构地区：[1]Centre of Excellence for Nanostructured Materials(CENMAT),INSTM,Department of Chemical and Pharmaceutical Sciences,University of Trieste,Via L.Giorgieri 1,34127,Trieste,Italy [2]Laboratoire LG2A-CNRS UMR 7378,Universitéde Picardie Jules Verne,33 rue Saint Leu-UFR des Sciences,80039,Amiens,France [3]Instituto de Carboquímica(CSIC),C/Miguel Luesma Castán 4,E-50018,Zaragoza,Spain [4]Renal Research Laboratory-Fondazione IRCCS Ca’Granda Ospedale Maggiore Policlinico,via Pace 9,20122,Milan,Italy [5]Dipartimento di Chimica,Materiali ed Ingegneria Chimica“G.Natta”,Politecnico di Milano,Via Mancinelli 7,20131,Milan,Italy [6]Unit of Nephrology,Dialysis and Renal Transplant Fondazione IRCCS Ca’Granda Ospedale Maggiore Policlinico,via della commenda 4,20122,Milan,Italy [7]Department of Clinical Sciences and Community Health,University of Milan,20122,Milan,Italy [8]Catalan Institute of Nanoscience and Nanotechnology(ICN2),CSIC and the Barcelona Institute of Science and Technology,Campus UAB,Bellaterra,08193,Barcelona,Spain

出　　处：《Nano Research》2021年第3期674-683,共10页纳米研究（英文版）

基　　金：J.M.G.-D.acknowledges Spanish Ministry of Science,Innovation and Universities for his Juan de la Cierva Incorporación research contract(No.IJCI-2016-27789);This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement No.734834(INFUSION)and No.734381(CARBO-IMmap);from MIUR.ICN2 is supported by the Severo Ochoa program from Spanish MINECO(No.SEV-2017-0706).

摘　　要：Carbon nanomaterials offer excellent prospects as therapeutic agents,and among them,graphene quantum dots(GQDs)have gained considerable interest thanks to their aqueous solubility and intrinsic fluorescence,which enable their possible use in theranostic approaches,if their biocompatibility and favorable pharmacokinetic are confirmed.We prepared ultra-small GQDs using an alternative,reproducible,top-down synthesis starting from graphene oxide with a nearly 100%conversion.The materials were tested to assess their safety,demonstrating good biocompatibility and ability in passing the ultrafiltration barrier using an in vitro model.This leads to renal excretion without affecting the kidneys.Moreover,we studied the GQDs in vivo biodistribution confirming their efficient renal clearance,and we demonstrated that the internalization mechanism into podocytes is caveolae-mediated.Therefore,considering the reported characteristics,it appears possible to vehiculate compounds to kidneys by means of GQDs,overcoming problems related to lysosomal degradation.

关 键 词：graphene quantum dots PODOCYTES BIODISTRIBUTION uptake pathway renal clearance NANOCARRIER 

分 类 号：R69[医药卫生—泌尿科学] TQ127.11[医药卫生—外科学]