海绵胶煤炱菌(竹燕窝)多糖的组成及体外抗氧化活性  

The Antioxidant Activity and Monosaccharide Composition Analysis of the Polysaccharide from Scorias spongiosa

作　　者：钟胜男 禹小波 胡烨 农向 ZHONG Shengnan;YU Xiaobo;HU Ye;NONG Xiang(Bamboo Diseases and Pest Control and Resources Development Key Laboratory of Sichuan Province,Leshan 614000,China;College of Life Science,Leshan Normal University,Leshan 614000,China;College of Food and Biological Engineering,Xihua University,Chengdu 610000,China)

机构地区：[1]竹类病虫防控与资源开发四川省重点实验室,四川乐山614000 [2]乐山师范学院生命科学学院,四川乐山614000 [3]西华大学食品与生物工程学院,四川成都610000

出　　处：《食品工业科技》2021年第6期62-66,共5页Science and Technology of Food Industry

基　　金：四川省科技厅应用基础项目(19YYJC303);四川省属高校科研创新团队项目(18TD0032);省重点实验室项目(ZL2019003);横向企业委托项目(LHX109796)。

摘　　要：本文研究海绵胶煤炱菌(竹燕窝)多糖的单糖组成及体外抗氧化活性。采用超声波辅助提取方法提取海绵胶煤炱菌多糖,通过高效液相色谱(HPLC)分析单糖组成,并通过DPPH·、ABTS^(+)·和·OH的清除能力评价其抗氧化能力。结果表明,海绵胶煤炱菌多糖得率为9.61%,总糖含量为70.92±1.530 g/100 g,单糖组成为甘露糖、葡萄糖、半乳糖,摩尔比为1.35∶212∶1。体外抗氧化实验表明,海绵胶煤炱菌多糖对DPPH·、ABTS^(+)·和·OH清除能力与多糖浓度成正相关,其IC_(50)分别为(0.796±0.002)、(0.923±0.012)和(1.993±0.026)mg/mL。本实验证明了海绵胶煤炱菌多糖是一种天然的抗氧化资源,可以进一步研究海绵胶煤炱菌多糖的其他生物活性,为深度开发海绵胶煤炱菌多糖在食品、医药和化妆品等领域中的应用提供依据。The monosaccharide composition and antioxidant activity of Scorias spongiosa polysaccharide(SSP)were analyzed.The aim of this study was to investigate the polysaccharide contents and antioxidant activities of Scorias spongiosa extract.The SSP obtained through ultrasound-assisted extraction was characterized,and its monosaccharide composition was studied by high-performance liquid chromatography.The antioxidant capacity was evaluated by 1,1-diphenyl-2-picryl-hydrazyl(DPPH),2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid)(ABTS^(+)·),and·OH.The polysaccharide extraction rate of Scorias spongiosa was 9.61%.The total sugar content was 70.92±1.530 g/100 g,which was mainly composed of glucose,mannose,and galactose in the molar ratio of 1.35∶212∶1.SSP had a relatively high antioxidant activity as revealed by scavenging ability tests on DPPH·,ABTS^(+)·,and·OH.Antioxidant assays showed the enhanced potency of SSP with its half maximal inhibitory concentration values of(0.796±0.002),(0.923±0.012),and(1.993±0.026)mg/mL for DPPH·,ABTS^(+)·,and·OH,respectively.This investigation shows that polysaccharide extracts obtained from Scorias spongiosa might be used as bioactive natural sources in the food,and cosmetic industries.Furthermore,we could further study the other biological activities of the polysaccharides.

关 键 词：海绵胶煤炱菌 多糖 HPLC 单糖 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]