小麦籽粒植酸含量聚类及相关基因位点研究  

Cluster Analysis and Association Mapping of Phytic Acid Content among Wheat Cultivars

作　　者：陈广凤[1] 李冬梅[1] 邓志英 冯建英[1] 郑世英[1] 郑芳[1] 吴秀芬[1] 田纪春[1] CHEN Guang-feng;LI Dong-mei;DENG Zhi-ying;FENG Jian-ying;ZHENG Shi-ying;ZHENG Fang;WU Xiu-fen;TIAN Ji-chun(Dezhou University,College of Ecology,Resources and Environment,Dezhou,Shandong 253000,China;Agronomy College of Shandong Agricultural University,Stage Key Laboratory of Crop Biology,Group of Wheat Quality Breeding,Tai’an,Shandong 271018,China)

机构地区：[1]德州学院生态与资源环境学院,山东德州253000 [2]山东农业大学农学院,小麦品质育种研究室,作物生物学国家重点实验室,山东泰安271018

出　　处：《粮油食品科技》2021年第2期25-33,共9页Science and Technology of Cereals,Oils and Foods

基　　金：山东省自然基金(ZR20171702170343);山东省高等学校科技计划项目(J17KB116;J17KA148);德州学院横向课题(HXKT2020042);山东省农业良种工程(2019LZGC017);国家自然科学基金(31871613)。

摘　　要：以205份中国冬麦区小麦品种(系)为材料,分析其籽粒植酸含量做聚类分析并解析其关联位点。结果表明,两个环境群体植酸含量平均值为4.99 g/kg和3.31 g/kg,变幅分别为17.90 g/kg和10.20 g/kg,变异范围较大。聚类分析将群体材料聚为6类,类间植酸含量差异均显著。推广品种或骨干亲本中,B54、B40、B86和B126植酸含量最低,可做为低植酸小麦育种中重要的亲本。高代品系B131、B141、B181和B149植酸含量最低,结合优异农艺性状,有望选出低值酸含量功能性小麦新品种。共检测到36个与小麦籽粒植酸含量显著关联的单核苷酸多态性(SNP)标记关联位点(P<0.001),分布在2B、3A、3B、3D、6A和6B染色体上,单个关联位点表型变异贡献率为5.73%~9.69%;鉴定出了低值酸含量基因的优异等位变异如Tdurum_contig35799_208-G、Excalibur_c96134_152-C和Tdurum_contig43538_1687-A等对低植酸含量效应较大。In this study,205 elite wheat lines from winter wheat region of China were used as materials to analyze the phytic acid content,and the cluster analysis and related was further conducted.The results showed that the average Phytic acid content of the two environmental groups was 4.99 g/kg and 3.31 g/kg,and the variation range was 17.90 g/kg and 10.20 g/kg,respectively.The variation range was relatively large.The results of cluster analysis showed that there were significant differences in Phytic acid content among 6 groups.B54,B40,B86 and B126 have the lowest phytic acid content among the extended varieties or backbone parents,which can be used as important parents in low Phytic acid wheat breeding.The higher generation lines B131,B141,B181 and B149 had the lowest phytic acid content.Combined with excellent agronomic traits,it is expected to select new functional wheat varieties with low Phytic acid content.A total of 36 single nucleotide polymorphism(SNP)markers associated with phytic acid content in wheat grains were detected(P<0.001),which were distributed on chromosomes 2B,3A,3B,3D,6A and 6B.The contribution rate of phenotypic variation of a single Association site was 5.73%~9.69%.Excellent alleles of genes with low Phytic acid content were identified such as tdurum,were identified_contig35799_208-G、Excalibur_c96134_152-C and tdurum_contig43538_1687-A,etc.which had a greater effect on low Phytic acid content.

关 键 词：普通小麦 植酸含量 聚类分析 营养品质 

分 类 号：TS202.1[轻工技术与工程—食品科学]