压榨法制备苹果籽油及其毒理学  

The Preparation of Apple Seed Oil by Pressing and Its Toxicology

作　　者：刘少阳[1] 丁秀玲 LIU Shaoyang;DING Xiuling(Food and Nutrition Department,Luohe Medical Collage,Henan Engineering Research Center of Leisure Food,Luohe 462000;Statistical Department,Liangyuan District,Shangqiu City,Shangqiu 476000)

机构地区：[1]漯河医学高等专科学校食品营养系,河南省休闲食品工程技术研究中心,漯河462000 [2]商丘市梁园区统计局,商丘476000

出　　处：《食品工业》2021年第1期339-342,共4页The Food Industry

基　　金：漯河医学高等专科学校2015年度基础科学研究项目(2015-S-LMC012)。

摘　　要：论文结合生产实际,尝试收集苹果籽,探讨苹果籽油工业化生产的可行方法,并通过毒理学研究评价其安全性。在生产线上通过筛选、清洗、干燥等流程,共收集苹果籽68.5 kg,采用压榨法制备苹果籽油9.75 kg,出油率为14.23%;通过小鼠急性毒性实验、小鼠精子畸形的遗传毒性实验和30 d喂养实验对其进行毒理学评价,结果显示:苹果籽油对小鼠的急性经口LD_(50)大于20 g/kg BW,判属无毒类;小鼠精子畸形遗传毒性实验为阴性,表明该受试物无致突变作用;在大鼠30 d喂养实验中未见动物健康状况和器官组织形态的异常变化;据此初步估计苹果籽油最大无作用剂量大于5.0 g/kg BW,具有较高的食用安全性。Based on the actual production,this paper attempts to collect apple seeds,explores the feasible methods of industrialized production of apple seed oil,and studies its toxicological safety.A total of 68.5 kg of apple seeds were collected on the production line through screening,cleaning,drying,etc.,and 9.75 kg of the apple seed oil was prepared by pressing,with an oil yield of 14.23%;Mainly through the acute toxicity test,mice sperm abnormal genetic toxicity and the 30 d feeding test to determine the toxicology.According to the result of acute toxicity LD_(50) test in mice,the apple seed oil was nontoxicity for the LD_(50)>20 g/kg BW;Mice sperm abnormal genetic toxicity test was negative,indicating that the subjects of noninduced mutation;After 30 d feeding experiment of rats,there were no abnormal changes in the terms of health status,as well as organ and tissue morphology.Based on this preliminary estimation,the maximum non-action dose of apple seed oil was greater than 5.0 g/kg BW,which had a high edible safety.

关 键 词：苹果籽油 压榨法 工业化生产 毒理学 

分 类 号：R994.4[医药卫生—毒理学] TS225.19[医药卫生—药学]