响应面优化海鲜菇副产物黄酮类化合物提取工艺  

Optimization of Extraction Process for Flavonoids from the By-products of Hypsizygus Marmoreus by Response Surface Methodology

作　　者：赖谱富[1] 李怡彬[1] 翁敏劼[1] 汤葆莎[1] 吴俐[1] 陈君琛[1] LAI Pu-fu;LI Yi-bin;WENG Min-jie;TANG Bao-sha;WU Li;CHEN Jun-chen(Research Institute of Agri-Engineering Technology,Fujian Academy of Agricultural Sciences/AgriculturalProduct Processing Research Centre,Fujian Academy of Agricultural Sciences/National R&D Center ForEdible Fungi Processing Technology,Fuzhou,Fujian 350003,China)

机构地区：[1]福建省农业科学院农业工程技术研究所/福建省农业科学院农产品加工研究中心/国家食用菌加工技术研发分中心,福建福州350003

出　　处：《福建农业科技》2021年第2期1-6,共6页Fujian Agricultural Science and Technology

基　　金：国家重点研发计划项目(2018YFD0400203);福建省科技计划项目——省属公益类科研院所基本科研专项(2019R1032-10);福建省现代食用菌产业技术体系建设项目(闽财指〔2019〕897号);福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-3-5)。

摘　　要：利用响应面法对海鲜菇副产物黄酮类化合物的提取工艺进行优化。在单因素试验基础上,选择料液比、提取温度、提取时间、乙醇浓度为自变量,黄酮类化合物得率为响应值,利用响应面分析法研究各自变量交互作用及其对黄酮类化合物得率的影响,模拟得到二次多项式回归方程的预测模型。结果表明:海鲜菇副产物黄酮类化合物最佳提取工艺条件为料液比1∶28、提取温度50℃、提取时间1.4 h、乙醇浓度58%、振荡速率120 r·min^(-1),在此工艺条件下黄酮类化合物得率为3.41 mg·g^(-1)。The response surface methodology was used to optimize the extraction process of flavonoids from the by-products of Hypsizygus marmoreus.On the basis of the single factor experiment,the solid-liquid ratio,extraction temperature,extraction time and ethanol concentration were selected as the independent variables,and the yield of flavonoids was selected as the response value.Then,the interaction of each independent variable and its effect on the yield of flavonoids were studied by using the response surface method,and the prediction model of quadratic polynomial regression equation was obtained by simulation.The results showed that the optimal extraction conditions of the flavonoids from the by-products of Hypsizygus marmoreus were as follows:the solid-liquid ratio of 1∶28,extraction temperature of 50℃,extraction time of 1.4 h,ethanol concentration of 58%,and oscillation rate of 120 r·min^(-1).Under these conditions,the yield of the flavonoids was 3.41 mg·g^(-1).

关 键 词：海鲜菇 副产物 黄酮类化合物 提取 响应面法 

分 类 号：TS255.5[轻工技术与工程—农产品加工及贮藏工程]