牛初乳中抗高血压因子对成年大鼠心肌细胞电压依懒性钙通道的影响  

The Effect of Anti-hypertension Factor in Bovine Colostrums on Intracellular Voltage Dependent Calcium Channel in Adult Rat Ventricular Myocytes

作　　者：滕刚[1] 任皓威[1] 赵添玉 商佳琦 刘宁[1] Teng Gang;Ren Haowei;Zhao Tianyu;Shang Jiaqi;Liu Ning(Key Lab of Dairy Science,Ministry of Education,College of Food Science,Northeast Agricultural University,Harbin 150030)

机构地区：[1]东北农业大学食品学院,乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国食品学报》2021年第3期102-106,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省科技攻关计划项目(GB01B407-02)。

摘　　要：目的:通过观察牛初乳中抗高血压因子(AFBC)对氯化钾诱发成年大鼠心肌细胞内游离钙浓度([Ca^(2+)]i)的影响,探究可能抑制大鼠心肌收缩的作用机制。方法:应用钙荧光指示剂Fura-2/AM负载急性分离的大鼠心肌细胞。结果:AFBC(0.5 mg/mL)对静息状态下心肌细胞内Ca^(2+)浓度无明显影响,可明显抑制高钾(50 mmol/L)引起的心肌细胞内Ca^(2+)浓度的升高。结论:AFBC可阻断成年大鼠的心肌细胞电压依赖性钙通道。Objective:To observe the effect of anti-hypertensive factor(AFBC)in bovine colostrum on the intracellular free calcium concentration([Ca^(2+)]i)induced by potassium chloride in adult rat cardiomyocytes,the possible mechanism of inhibiting myocardial contraction in rats was investigated.Methods:Using fluorescent properties Fura-2/AM at freshly isolated rat ventricular myocytes.Results:0.5 mg/mL AFBC had no significant effect on resting myocardisl cells[Ca^(2+)]i,but could depress the increase of ventricular myocytes[Ca^(2+)]i induced by 50 mmol/L KCl.Conclusion:AFBC can block the voltage-dependent calcium channel of ventricular myocytes in adult rat.

关 键 词：牛初乳 抗高血压 心肌细胞 细胞内游离钙浓度 FURA-2/AM 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] TS201.4[轻工技术与工程—食品科学与工程]