脂肪酶-蜗牛酶提取茶籽多糖及其抗氧化作用  

Extraction of Camellia oleifera seed polysaccharides by lipase-snailase and its antioxidant effect

作　　者：周丽明[1] 张勇[1] ZHOU Liming;ZHANG Yong(School of Life Science,Shangrao Normal University,Shangrao 334001,Jiangxi,China)

机构地区：[1]上饶师范学院生命科学学院,江西上饶334001

出　　处：《中国油脂》2021年第2期114-118,123,共6页China Oils and Fats

基　　金：江西省教育厅科学技术研究项目(GJJ161055)。

摘　　要：以油茶籽饼为原料,优化脂肪酶-蜗牛酶提取茶籽多糖的条件,探讨酶提茶籽多糖的抗氧化作用。通过单因素试验和正交试验分析酶解温度和p H、酶解时间、酶添加量、脂肪酶和蜗牛酶质量比对茶籽多糖得率的影响;测定和比较酶提茶籽多糖与水提茶籽多糖对O2-·、DPPH·、·OH、ABTS^(+)·4种自由基的清除率;采用Schaal烘箱法研究酶提茶籽多糖对油脂的抗氧化作用。结果表明:脂肪酶-蜗牛酶提取茶籽多糖的最佳工艺条件为酶解温度35℃、p H 7.0、酶解时间2.5 h、酶添加量2.0%、脂肪酶和蜗牛酶质量比2∶3,在此条件下茶籽多糖得率可达5.83%;酶提茶籽多糖的抗氧化作用强于水提茶籽多糖。酶提茶籽多糖具有作为油脂抗氧化剂的潜质。The conditions of extracting Camellia oleifera seed polysaccharides( COSP) from Camellia oleifera seed cake by lipase-snailase were optimized and the antioxidant effect of COSP was investigated.The effects of enzymolysis temperature and p H,enzymolysis time,enzyme dosage,mass ratio of lipase to snailase on yield of COSP were analysed by single factor experiment and orthogonal experiment. The scavenging rates of COSP extracted by lipase-snailase and water on O2^(-)·,DPPH·,·OH and ABTS^(+)· were determined and compared. The antioxidant effects of COSP extracted by lipase-snailase on oils were investigated by Schaal oven method. The results showed that the optimal conditions of COSP extracted by lipase-snailase were obtained as follows: enzymolysis temperature 35 ℃, pH 7. 0,enzymolysis time 2. 5 h,enzyme dosage 2. 0% and mass ratio of lipase to snailase 2 ∶ 3. Under these conditions,the yield of COSP was 5. 83%. The antioxidant effect of COSP extracted by lipase-snailase was better than that of COSP extracted by water. COSP extracted by lipase-snailase had potential as antioxidant for oils.

关 键 词：茶籽多糖 脂肪酶 蜗牛酶 抗氧化 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] O629.1[轻工技术与工程—食品科学与工程]