海参酶解物促SD大鼠皮肤创面愈合效果的研究  

Effect of sea cucumber enzymatic hydrolysate on skin wound healing in SD rats

作　　者：程敏君 胡锦华[1] 唐雪[2] 杨亭亭 周鹏[1] 胡炜 CHENG Minjun;HU Jinhua;TANG Xue;YANG Tingting;ZHOU Peng;HU Wei(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Shandong Homey Aquatic Development Co.Ltd.,Weihai 264200,China)

机构地区：[1]食品科学与技术国家重点实验室(江南大学),江苏无锡214122 [2]江南大学食品学院,江苏无锡214122 [3]山东好当家海洋发展股份有限公司,山东威海264200

出　　处：《食品与发酵工业》2021年第7期94-101,共8页Food and Fermentation Industries

基　　金：食品科学与技术国家一级学科计划项目(JUFSTR20180201)。

摘　　要：目的:为探究海参酶解物(sea cucumber enzymatic hydrolysate,SCH)对SD大鼠皮肤创面愈合的影响,为SCH应用于创面愈合提供理论依据。方法:设立模型组、3个SCH剂量组和阳性组,建立大鼠全层皮肤切除模型,于术后第4、8和12天分批处死。通过计算创面愈合率、进行组织切片伊红染色(hematoxylin-eosin,H&E)和马松三色染色(Masson’s trichrome method,Masson),以及结合血液中白细胞介素-8(interleukin-8,IL-8)和白细胞介素-10(interleukin-10、IL-10)、创面处羟脯氨酸(hydroxyproline,Hyp)和血管内皮生长因子(vascular endothelial growth factor,VEGF)、表皮生长因子(epidermal growth factor,EGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的含量,综合分析SCH对创面愈合的影响。结果表明:SCH各剂量组的创面愈合率均高于模型组;H&E结果显示SCH可以调节炎性细胞浸润、促进血管新生,且中剂量组表皮恢复最快;IL-8和IL-10结果显示SCH可通过调节其分泌量来缩短炎症反应时间;Masson染色观察到SCH显著促进创面处胶原纤维的生成,且Hyp含量相应增加。此外,SCH促创面愈合机制可能与生长因子分泌量显著增加有关。结论:SCH具有促SD大鼠创面愈合的功效,中剂量组(0.50 g/kg)的促愈合效果最佳。The impact of sea cucumber hydrolysate(SCH)on skin wound healing in SD rats was investigated,aiming to provide basis for the application of SCH in wound healing.A rat model of full-thickness cutaneous wounds was established in a model group,three SCH dose groups and a positive group,and the rats were sacrificed in batches on the 4 th,8 th and 12 nd day after surgery.The effect of SCH on wound healing was comprehensively analyzed in combination with the results of wound healing rate,observation of tissues stained with hematoxylin-eosin(H&E)and Masson’s trichrome method(Masson),changes of interleukin-8(IL-8)and interleukin-10(IL-10)in the blood,detection of hydroxyproline(Hyp),vascular endothelial growth factor(VEGF),epidermal growth factor(EGF)and basic fibroblast growth factor(bFGF)in wounds.Results showed that the wound healing rate of each dose group of SCH was faster than that of the model group.H&E results showed that SCH could regulate the infiltration of inflammatory cells and promote angiogenesis,and the epidermis recovered fastest in the medium dose group.Based on the results of IL-8 and IL-10,SCH could regulate their secretion to reduce the inflammatory reaction duration.Collagen fibers formation in the wound significantly promoted by SCH was observed in Masson’s trichrome stained tissues,and Hyp content increased accordingly.In addition,the mechanism of SCH wound healing may be related to the significant increase of growth factors secretion.The prepared SCH could effectively promote wound healing in SD rats,with best performance observed in the medium dose group(0.50 g/kg).

关 键 词：海参 酶解物 皮肤创面愈合 

分 类 号：TS201.2[轻工技术与工程—食品科学] TQ460.1[轻工技术与工程—食品科学与工程]