在体单向肠灌流模型检验大豆皂苷苷元的肠吸收特性  

Determination of Soyasapogenols Absorption Characteristics in Rat Gut Using in Situ Single-pass Intestinal Perfusion Model

作　　者：潘承慧 齐东月 赵大云[1] PAN Chenghui;QI Dongyue;ZHAO Dayun(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《食品工业科技》2021年第10期336-342,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金(31972069)。

摘　　要：目的:探究大豆皂苷苷元A和B的肠吸收特性。方法:建立HPLC色谱分析方法测定灌流液的苷元浓度,基于大鼠肠段的形态学变化考察在体单向肠灌流模型的可靠性,分别研究不同肠段(十二指肠、空肠、回肠、结肠)、苷元剂量和胆汁对大豆皂苷苷元肠吸收特性的影响。结果:大豆皂苷苷元在不同肠段的表观渗透速率(P_(app))和吸收速率常数(K_a):空肠>十二指肠>回肠>结肠,苷元A在空肠段的P_(app)和K_a为15.34×10^(-5) cm/s和3.06×10^(-5) s^(-1)(剂量50 mg/L),苷元B的P_(app)和K_a为11.36×10^(-5) cm/s和2.74×10^(-5) s^(-1)(剂量50 mg/L);苷元A的吸收有显著浓度依赖性(P<0.05),而苷元B浓度依赖性不明显(P>0.05);有胆汁时,苷元A和B在空肠段的P_(app)分别为16.56×10^(-5)和12.01×10^(-5)cm/s, K_a分别为3.06×10^(-5)和2.97×10^(-5)s^(-1),均显著高于无胆汁组(P<0.05),胆汁的存在一定程度提高了肠部上端苷元的吸收。结论:大豆皂苷苷元主要吸收部位为空肠,苷元A吸收机制为被动扩散,胆汁的参与促进了吸收作用。Objective: To investigate the absorption characteristics of soyasapogenol A and B. Methods: The effects of absorption sites(duodenum, jejunum, ileum, and colon), concentration and bile on the intestinal absorption of soyasapogenol were determined using HPLC analysis and in situ single-pass intestinal perfusion model. Results: The order of apparent absorption coefficient(Papp) and absorption rate constant(Ka) of soyasapogenol absorption sites were as followed: Jejunum>duodenum>ileum>colon. The Papp and Ka of soyasapogenol A at a concentration of 50 mg/L were15.34×10-5 cm/s and 3.06×10-5 s-1 at jejunum, while soyasapogenol B were 11.36×10-5 cm/s and 2.74×10-5 s-1. The absorption capacity of soyasapogenol A was significantly concentration-dependent(P<0.05) while soyasapogenol B was not significant(P>0.05). The Papp of soyasapogenol A and B with bile were 16.56×10-5 and 12.01×10-5 cm/s, and the Ka were 3.06×10-5 and 2.97×10-5 s-1, which was higher than those without bile significantly(P<0.05). The presence of bile improved the absorption capability at upper part of the intestine to some extent. Conclusion: The absorption site of soyasapogenol was mainly at jejunum. The absorption mechanism of soyasapogenol A was passive diffusion, and the bile tended to have a positive impact on its intestinal absorption.

关 键 词：大豆皂苷苷元 在体单向肠灌流 肠吸收 形态学变化 

分 类 号：TS201.4[轻工技术与工程—食品科学]