紫苏叶中甘油糖脂的抗氧化活性研究  

The study of antioxidant activities of glycoglycerolipids in leaves of Perilla frutescens

作　　者：訾玉祥 陆兆新[1] 吕风霞[1] 别小妹[1] 张充[1] 赵海珍[1] ZI Yuxiang;LU Zhaoxin;Lü Fengxia;BIE Xiaomei;ZHANG Chong;ZHAO Haizhen(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品科学技术学院,江苏南京210095

出　　处：《南京农业大学学报》2021年第3期561-567,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31771950,31301558);江苏高校优势学科建设工程资助项目。

摘　　要：[目的]本文旨在研究紫苏叶中3种不同分子结构甘油糖脂的抗氧化活性并初步探讨其活性构效关系。[方法]通过化学评价法评价甘油糖脂的2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、1,1-二苯基-2-三硝基苯肼(DPPH)、羟自由基(·OH)及超氧阴离子(O_(2)^(·-))清除能力并测定其还原力,采用细胞氧化损伤模型研究甘油糖脂对抗氧化酶活性的影响。[结果]从紫苏叶中分离纯化获得了3种甘油糖脂单体,即MGDG(C18∶3/C18∶3)、DGDG(C18∶3/C18∶3)和DGDG(C18∶3/C16∶0)。MGDG(C18∶3/C18∶3)和DGDG(C18∶3/C18∶3)的抗氧化能力强于DGDG(C18∶3/C16∶0)。3种甘油糖脂均可抑制ABTS自由基、DPPH自由基、·OH、O_(2)^(·-)的产生及提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性,且具有较强的还原力。[结论]3种甘油糖脂均具有抗氧化能力,但脂肪酰基组成不同的甘油糖脂具有不同的抗氧化能力,其中,脂酰基及糖基数量对甘油糖脂类化合物的抗氧化活性影响较大。[Objectives]This article aimed to study the antioxidant activities of three different molecular structures of glycoglycerolipids in leaves of Perilla frutescens and the structure-activity relationship was preliminarily discussed.[Methods]The scavenging abilities of 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid(ABTS),1,1-diphenyl-2-picrylhydrazyl(DPPH),hydroxyl radicals(·OH)and superoxide anion radicals O_(2)^(·-) were measured by chemical evaluation method,and the cell oxidative damage model was used to study the effects of glycoglycerolipids on the activities of antioxidant enzymes.[Results]Three glycoglycerolipids monomers were separated and purified from leaves of P.frutescens,including MGDG(C18∶3/C18∶3),DGDG(C18∶3/C18∶3)and DGDG(C18∶3/C16∶0).The antioxidant activities of MGDG(C18∶3/C18∶3)and DGDG(C18∶3/C18∶3)were higher than that of DGDG(C18∶3/C16∶0).All glycoglycerolipids presented strong reducing power,and they could not only inhibit the production of ABTS,DPPH,·OH and O_(2)^(·-),but also significantly enhance the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px).[Conclusions]Glycoglycerolipids with different fatty acyl composition presented different antioxidant activity,and the preliminary study of structure-activity relationship showed that the saturation degree of fatty acyl group and the number of sugar groups greatly affected the antioxidant activities of glycoglycerolipids.

关 键 词：紫苏 甘油糖脂 抗氧化活性 结构-活性关系 

分 类 号：TS201.2[轻工技术与工程—食品科学]