纤维素酶对柱花草青贮品质和营养成分的影响  

Effect of cellulase on the quality and nutrient composition of Stylosanthes guianensis silage

作　　者：张立冬 字学娟[2] 李茂[3] 吕仁龙 胡海超 唐军[3] 周汉林[3] ZHANG Lidong;ZI Xuejuan;LI Mao;LV Renlong;HU Haichao;TANG Jun;ZHOU Hanlin(College of Tropical Crops,Hainan University,Haikou 570228,China;College of Foristy,Hainan University,Danzhou 571737,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]海南大学热带作物学院,海口570228 [2]海南大学林学院,海南儋州571737 [3]中国热带农业科学院热带作物品种资源研究所,海口571101

出　　处：《黑龙江畜牧兽医》2021年第7期118-121,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：中国热带农业科学院基本科研业务费项目(1630032017033);农业农村部项目“热带亚热带可利用饲料资源质量安全评价”(2130109;12200012);滇桂黔石漠化地区特色作物产业发展关键技术集成示范项目。

摘　　要：为了探究纤维素酶对柱花草营养成分和青贮品质的影响,试验以热研2号柱花草为材料,设对照组(CK组)和4个纤维素酶添加组(S1~S4组),每组3个重复,对照组不添加纤维素酶,S1~S4组分别添加5,10,20,40 mg/kg纤维素酶,青贮30 d后取样分析营养成分和青贮品质。结果表明:S1~S4组的干物质含量和粗蛋白含量均显著高于对照组(P<0.05),中性洗涤纤维和酸性洗涤纤维含量均显著低于对照组(P<0.05)。与对照组相比,添加纤维素酶的S1~S4组能显著降低柱花草青贮饲料的pH值(P<0.05),S1~S4组的乳酸含量均显著高于对照组(P<0.05)。与对照组相比,S4组(添加40 mg/kg纤维素酶)的干物质、粗蛋白和乳酸含量分别提升了10%、12%和69%,中性洗涤纤维、酸性洗涤纤维含量及pH值降低了10%、8%和15%。说明添加纤维素酶可以有效改善柱花草青贮品质并提升营养价值,添加40 mg/kg纤维素酶较为适合。In order to explore the effect of cellulase on the quality and nutritional composition of Stylosanthes guianensis silage, Stylosanthes guianensis cv. Reyan No.2 was divided into control(CK group) group and 4 cellulase addition groups(S1-S4 groups) with 3 repeats in each group. The control group did not add cellulase, and the S1-S4 groups was supplemented with 5, 10, 20 and 40 mg/kg cellulase. After 30 days of silage, the silage quality and nutritional composition were analyzed. The results showed that the dry matter content and crude protein content in S1-S4 groups were significantly higher than those of CK group(P<0.05), while the contents of neutral detergent fiber and acid detergent fiber were significantly lower than those in the CK group(P<0.05). The pH value of Stylosanthes guianensis silage in the S1-S4 groups were significantly lower than that in CK group(P<0.05), and the lactic acid content in the S1-S4 groups were significantly higher than that in the CK(P<0.05). Compared with the control group, the dry matter, crude protein and lactic acid content in the S4 group(adding 40 mg/kg cellulase) increased by 10%, 12%, and 69%, respectively, and the content of neutral detergent fiber, acid detergent fiber and pH decreased by 10%, 8% and 15%, respectively. The results indicated that the addition of cellulase to Stylosanthes guianensis could effectively improve the quality and nutritional value of silage, and the addition of 40 mg/kg cellulase was more suitable.

关 键 词：热研2号 柱花草 纤维素酶 营养成分 青贮品质 

分 类 号：S816.9[农业科学—饲料科学]