基于HS-SPME-GC-MS法分析大麦幼苗中的挥发性成分  

Analysis on Volatile Components in Barley Grass by HS-SPME-GC-MS

作　　者：李静[1,2] 张述伟 周龙华 徐红卫 陆瑞菊[1,2] 刘成洪 LI Jing;ZHANG Shu-wei;ZHOU Long-hua;XU Hong-wei;LU Rui-ju;LIU Cheng-hong(Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China)

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106

出　　处：《食品研究与开发》2021年第10期148-153,共6页Food Research and Development

基　　金：国家大麦青稞产业技术体系项目(CARS-05-01A-02);上海市科技兴农重点攻关项目(2017-02-08-00-08-F000074);上海市科学技术委员会科研计划项目(19ZR1417000);上海市农业科学院农业科技创新支撑领域研究专项(KJZC202008)。

摘　　要：为开发大麦资源,明确大麦幼苗中的主要挥发性成分,采用顶空固相微萃取(headspace solid phase microextraction,HS-SPME)和气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用技术分析两种大麦幼苗中的挥发性成分和真空干燥后大麦幼苗中挥发性成分的变化。从两种大麦鲜苗中分别鉴定出36种和52种成分,大麦干苗中分别鉴定出35种和34种成分。两个品种大麦幼苗中的挥发性成分种类基本相似,但其主要挥发性成分和含量存在差别。大麦幼苗中的挥发成分包括醛、酮、醇、酸、酯、烃、酚、醚、呋喃、吡嗪、吡咯以及吲哚等多类物质。真空干燥后大麦幼苗中的挥发性成分种类减少,变化较大,原有的醛类成分减少,吡嗪类成分增加。To develop barley grass resources,volatile compounds in two barley grass and the change after vacu-um drying were investigated by headspace solid phase micro-extraction(HS-SPME)followed by gas chro-matography-mass spectrometry(GC-MS).36 and 52 kinds of components were identified from the two fresh barley grass,while 35 and 34 components were identified from dried barley grass.The types of volatile compo-nents in the two varieties of barley grass were similar,but their main volatile components and content were dif-ferent.Volatile components in the barley grass included aldehydes,ketones,alcohols,acids,esters,hydrocar-bons,phenols,ethers,furans,pyrazines,pyrroles and indole.After vacuum drying,the types of volatile com-ponents in fresh barley grass decreased greatly,the original aldehyde components decreased,and the pyrazine components increased.

关 键 词：大麦幼苗 顶空固相微萃取 真空干燥 气质联用 挥发性成分 

分 类 号：O657.63[理学—分析化学] TS210.7[理学—化学]