液相色谱–串联质谱法测定谷类食品中5种合成着色剂  

Determination of Five Synthetic Colorants in Grain Food by High Performance Liquid Chromatography-tandem Mass Spectrum

作　　者：张春娥 廖若宇 孙悦 刘新保 田建文[2] ZHANG Chun-e;LIAO Ruo-yu;SUN Yue;LIU Xin-bao;TIAN Jian-wen(Ningxia Hui Autonomous Region Grain and Oil Product Quality Inspection Center,Yinchuan,Ningxia 750001,China;Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan,Ningxia 750001,China)

机构地区：[1]宁夏回族自治区粮油产品质量检测中心,宁夏银川750001 [2]宁夏农林科学院,宁夏银川750001

出　　处：《粮油食品科技》2021年第3期163-169,共7页Science and Technology of Cereals,Oils and Foods

基　　金：宁夏回族自治区重点研发计划(2018BEG03070)。

摘　　要：通过添加食用小苏打的甲醇水溶液提取谷类食品中的合成着色剂,采用超高效液相色谱–串联质谱技术测定加标回收率,考察食用小苏打在谷类食品中对日落黄、苋菜红、胭脂红、诱惑红和亮蓝五种合成着色剂的萃取效果。试验样品经70 mL的40%甲醇水加1.00 g食用小苏打涡混提取后,通过固相萃取柱Strata X-AW SPE净化,结果显示,在0.1~1.4μg/mL范围内呈良好线性关系,五种合成着色剂的检出限范围为0.07~0.50μg/g。不同食品基质和不同加标水平条件(1、2、10μg/g)下,5种合成着色剂的加标回收率范围均在80%~110%间,相对标准偏差(RSD)范围为0.7%~5.1%(n=6),满足GB/T27404—2008《实验室质量控制规范食品理化检测》对回收率的规定要求。A new application of baking soda was developed,the synthetic colorant in cereal was extracted by adding with methanol solution in this paper.To develop new uses of edible bicarbonate,synthetic colorants were extracted from cereals by adding methanol water solution of edible bicarbonate in this paper,Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to test standard recovery.The extraction effect of edible bicarbonate of soda on five synthetic colorants of sunset yellow,amaranth red,carmine red,alluvium red and bright blue in cereals was investigated.The sample was extracted with 70 mL 40%methanol water vortexed with 1 g edible baking soda,purified with Strata X-AW SPE column and determined.The results showed that,a good linearity was observed between 0.1 and 1.4μg/mL,and for the 5 synthetic colorants,the limit of detection was between 0.07 and 0.50μg/g.The recoveries of the 5 synthetic colorants of different food substrates at different spiked levels(1,2 and 10μg/g)were all found to be in the recovery range specified in GB/T27404.The recoveries for the 5 synthetic colorants were found between 80.0%and 110%with RSD from 0.7%to 5.1%(n=6).Hence,baking soda added to 40%methanol water solution can be used to extract synthetic colorants from grain products with simple operation,accurate results,high efficiency and environmental protection.

关 键 词：合成着色剂 谷类食品 超高效液相色谱–串联质谱法 食用小苏打 

分 类 号：TS202.3[轻工技术与工程—食品科学]