复合诱变选育壳聚糖酶高产菌种及产酶条件优化  

Breeding of high-yield chitosanase strain by compound mutation and optimization of enzyme production conditions

作　　者：王俊芳[1] 张震 申梦雨 王刚[1] WANG Junfang;ZHANG Zhen;SHEN Mengyu;WANG Gang(College of Life Science,Henan University,Kaifeng 475004,China)

机构地区：[1]河南大学生命科学学院,河南开封475004

出　　处：《中国酿造》2021年第5期108-112,共5页China Brewing

基　　金：河南省科技厅基础与前沿研究计划项目(152300410063)。

摘　　要：以具有产壳聚糖酶能力的枯草芽孢杆菌(Bacillus subtilis)G10为出发菌,利用紫外和微波对菌株G10进行复合诱变,选育壳聚糖酶高产菌株,并采用正交试验设计对突变株产酶条件进行优化。结果选育出一株产酶活相对较高的突变株W1-32,优化后的产酶条件为果糖1.3%,胶体壳聚糖0.5%,酵母粉2.0%,MgSO_(4)·7H_(2)O 0.3%,初始pH 7.2,温度28℃,转速200 r/min。在此优化条件下,菌株W1-32产壳聚糖酶活为11.82 U/mL,是出发菌株G10的6.9倍。该研究为菌种的选育和进一步研究应用提供理论依据。Using Bacillus subtilis strain G10 with chitosanase-producing ability as the starting strain,the high-yield chitosanase strain was bred by ultraviolet mutagenesis and microwave mutagenesis,and the culture conditions of the mutant strain producing chitosanase were optimized by orthogonal tests design.The mutant strain W1-32 with relatively high chitosanase activity was screened,and the optimized culture conditions for high-yield chitosanase were fructose 1.3%,colloidal chitosan 0.5%,yeast powder 2.0%,MgSO_(4)7H_(2)O 0.3%,initial pH 7.2,temperature 28℃and rotation speed 200 r/min.Under the optimal conditions,the chitosan activity pooduced by strain W1-32 was 11.82 U/ml,which was 6.9 times of that of the starting strain G10.The study provided a theoretical basis for the breeding of strains and further application study.

关 键 词：枯草芽孢杆菌 壳聚糖酶 选育 紫外诱变 微波诱变 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]