液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素  

Simultaneous Detection of Multi-mycotoxins in Edible Oil Using Liquid Chromatography Tandem Mass Spectrometry

作　　者：杨帅 杨永坛 穆蕾 杨霞 杨悠悠 罗云敬[1] Yang Shuai;Yang Yongtan;Mu Lei;Yang Xia;Yang Youyou;Luo Yunjing(College of Life Science and Bioengineering,Beijing University of Tecnology,Bejing 100014;COFCO Nutrition&Health Research Institute,Beijing Key Laboratory of Nutrition Health and Food Safety,Beijing 102209;Agilent Technologies Inc.,Shanghai 200131)

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]中粮营养健康研究院营养健康与食品安全北京市重点实验室,北京102209 [3]安捷伦科技有限公司,上海200131

出　　处：《中国食品学报》2021年第5期309-316,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2017YFC1601603)。

摘　　要：基于分步液液提取以及脂质净化柱净化的样品前处理方式,建立同时测定食用植物油中28种真菌毒素的高效液相色谱-串联质谱的测定方法。28种真菌毒素的定量限范围0.07~1.25μg/kg,三水平加标回收率范围60%~120%,峰面积相对标准偏差小于12.5%,均满足国家标准GB/T 27404-2008《实验室质量控制规范食品理化检测》中实验室质量控制规范以及真菌毒素限量标准的要求。应用本方法对市售食用植物油样品进行真菌毒素的风险监测,结果显示:黄曲霉毒素、玉米赤霉烯酮及其衍生物、杂色曲霉素、T-2毒素有不同程度的检出。其中,花生油中主要的真菌毒素种类为黄曲霉毒素、杂色曲霉素以及玉米赤霉烯酮;菜籽油和玉米油中主要的真菌毒素种类为玉米赤霉烯酮及其衍生物。A method for simultaneous detection of 28 mycotoxins in edible oil was established by ultra performance liquid chromatography-tandem mass spectrometry.Sample preparation was fulfilled through two-step liquid-liquid extraction and solid-phase extraction sequentially.The methodological verification results showed that the limits of quantification(S/N,10)ranged from 0.07μg/kg to 1.25μg/kg,which were far less than the maximum levels of mycotoxins in foods.In addition,the recoveries of the three-level spiked samples were between 60%and 120%,and the relative standard deviations were less than 12.5%,which could meet the quality control specifications required in the national standard GB/T 27404-2008 Criterion on quality control of laboratories-Chemical testing of food.Using this method,risk monitoring of mycotoxins in the edible oils was executed,showing that aflatoxins,zearalenones and derivatives,sterigmatocysin and T-2 could possibly contaminated edible oils.In peanut oils,aflatoxins,zearalenone and sterigmatocysin were the main mycotoxins while zearalenone and its derivatives were the main mycotoxins in both rapeseed oils and corn oils.

关 键 词：真菌毒素 脂质净化 食用油 风险监测 

分 类 号：O657.63[理学—分析化学] TS225.1[理学—化学]