桦褐孔菌多糖的分离纯化及其抗氧化活性测定  

Isolation,Purification and Antioxidant Activity of Inonotus obliquus Polysaccharide

作　　者：李欣欣 李文香[1] LI Xinxin;LI Wenxiang(School of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《食品工业科技》2021年第11期192-197,共6页Science and Technology of Food Industry

基　　金：山东省现代农业产业技术体系食用菌创新团队建设专项基金(SDAIT-07-07)。

摘　　要：以桦褐孔菌为原料,测定其化学组分,并采用水提醇沉法提取桦褐孔菌粗多糖(IOP)。将获取的桦褐孔菌粗多糖进行脱蛋白、脱色素的初步纯化,然后利用DEAE-52纤维素层析柱及Sephadex-100凝胶柱对多糖提取液进行分离纯化,通过抗氧化活性筛选出活性较高的多糖组分,并通过高效液相色谱分析多糖组分的纯度。实验结果表明,桦褐孔菌子实体的化学成分组成丰富,其中多糖13.10%±0.31%、还原糖4.21%±0.40%、蛋白质2.70%±0.71%、灰分9.60%±0.31%。通过对桦褐孔菌粗多糖脱蛋白、脱色素的试验方法进行筛选,得到聚酰胺层析法为最佳的脱除方法。蛋白质的脱除率为93.1%、脱色率为75.8%,多糖的保留率为90.3%。IOP经过DEAE-52纤维素柱层析后得到四个单糖组分:IOP-1、IOP-2、IOP-3、IOP-4。对四个多糖组分进行抗氧化实验发现,IOP-2对DPPH自由基的清除率最高,清除率为81.9%。IOP-2经过Sephadex-100层析柱后获得两个多糖组分:IOP-2a、IOP-2b。对比其抗氧化活性,筛选出活性较高的多糖组分为IOP-2a。采用高效液相色谱法鉴定多糖组分IOP-2a,只检查出一个对称峰,说明IOP-2a为均一性多糖。The chemical compositions of Inonotus obliquus were analyzed and crude polysaccharides(IOP)were isolated from Inonotus obliquus by water extraction.The component of polysaccharides were purified by DEAE-52,and Sephadex G-100 column chromatography,and the highly active polysaccharide component was screened out through antioxidant activity,and the purity of the polysaccharide component was analyzed by high-performance liquid chromatography.The experimental results showed that the chemical composition of Inonotus obliquus was rich,including polysaccharide 13.10%±0.31%,reducing sugar 4.21%±0.40%,protein 2.70%±0.71%,ash 9.60%±0.31%.After IOP undergoes DEAE-52 cellulose column chromatography,four monosaccharide components were obtained:IOP-1,IOP-2,IOP-3,and IOP-4.Antioxidation experiments on four polysaccharide components found that IOP-2 had the highest scavenging rate for DPPH free radicals.After passing through Sephadex-100 chromatography column,IOP-2 obtained two polysaccharide components:IOP-2a and IOP-2b.Compared with its antioxidant activity,the polysaccharide component with higher activity was selected as IOP-2a.Using high performance liquid chromatography to identify the polysaccharide component IOP-2a,only one symmetrical peak was detected,indicating that IOP-2a was a homogeneous polysaccharide.

关 键 词：桦褐孔菌 多糖 分离纯化 抗氧化 高效液相色谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]