基于黄嘌呤氧化酶活性抑制和斑马鱼高尿酸血症模型的降尿酸功效食药材筛选  

Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model

作　　者：张瑛毓 普布多吉 卢聪 王凤忠 ZHANG Yingyu;PUBU Duoji;LU Cong;WANG Fengzhong(Institute of Food Science and Technology,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,China;Institute of Food Science and Technology CAAS,Beijing 100193,China)

机构地区：[1]西藏农牧科学院农产品开发与食品科学研究所,西藏拉萨850000 [2]中国农业科学院农产品加工研究所,北京100193

出　　处：《食品工业科技》2021年第12期334-339,共6页Science and Technology of Food Industry

基　　金：中国农业科学院创新工程(125161015000150013);西藏自治区财政专项资金项目(XZNKY-2019-C-050);西藏自治区自然基金项目(XZ2018ZRG-60(Z));中央级公益性科研院所基本科研业务费专项(S2020JBKY-20);中国农科院科技创新工程协同创新任务资助(CAAS-XTCX2018020-15)。

摘　　要：结合体外(黄嘌呤氧化酶活性抑制)和体内(斑马鱼高尿酸血症模型)方法,对15种食药材乙醇粗提物的降尿酸活性进行筛选。体外实验设置空白组、酶反应组、抑制剂组、对照组,酶标仪295 nm测定吸光度,计算15种食药材对黄嘌呤氧化酶(XOD)活性的抑制率;体内实验随机选取受精后第5 d(5dpf)的斑马鱼,设置空白组、模型组(200μmol/L PO+10μmol/L XSS)、食药材给药组(200μmol/L PO+10μmol/L XSS+不同浓度提取物)、阳性对照组(200μmol/L PO+10μmol/L XSS+APL 2 mmol/L),水溶浸泡,28.5℃培养箱中孵育24 h,测定尿酸含量,对具有良好XOD抑制活性的食药材做进一步降尿酸活性验证。体外试验结果表明,15种乙醇粗提物均具有XOD抑制活性,其中8种在400μg/mL时抑制率达到50%以上,分别为:红景天、兔耳草、牡丹皮、败酱草、黄柏、绿萝花、决明子、雪菊。体内试验结果表明,8种处理组尿酸水平与模型组相比显著降低(P<0.05或P<0.01),均显示出降尿酸活性。Through the combination of in vitro(the xanthine oxidase inhibitory activity)and in vivo(a zebrafish hyperuricemia model)methods,the uric acid-lowering activity of 15 edible herbs crude ethanol extracts was screened.In vitro experiment set up blank group,enzyme reaction group,inhibitor group and control group.The absorbance at 295 nm with microplate reader was measured.In vivo experiment 5 days post fertilization zebrafish larvae with normal development were randomly divided into blank group,model group(200μmol/L PO+10μmol/L XSS),edible herbs group(200μmol/L PO+10μmol/L XSS+extracts of different concentrations)and positive control group(200μmol/L PO+10μmol/L XSS+APL 2 mmol/L).All groups were cultivated at 28.5℃for 24 h to determine the uric acid contents and further verify the uric acid-lowering activity.In vitro experiment showed that 15 ethanol extracts were inhibitory,with 8 having greater than 50%inhibitory rate at 400μm/mL.The most active one was the ethanol extracts of the Rhodiola crenulate,which was followed closely by Lagotis brevituba,Poeania suffruticosa,Patrinia scabiosaefolia,Phellodendron chinense,Scindapsus aureus,Cassia tora and Coreopsis tinctorial.In vivo experiment showed that the uric acid level of all these eight extract groups were significantly lower than the model group(P<0.05 or P<0.01).

关 键 词：降尿酸 黄嘌呤氧化酶 斑马鱼高尿酸血症模型 活性筛选 食药材 

分 类 号：R151.3[医药卫生—营养与食品卫生学]