基于新型标记材料的免疫分析技术在真菌毒素检测中应用的研究进展  

Research Progress on Application of New Labeling Materials Based Immunoassay on the Detection of Mycotoxin

作　　者：胡高爽 吴天琪 苏丹 高娟娟 陈永媛 韩雪[1] 郝建雄[1] 李娜 郭峰 高山 HU Gaoshuang;WU Tianqi;SU Dan;GAO Juanjuan;CHEN Yongyuan;HAN Xue;HAO Jianxiong;LI Na;GUO Feng;GAO Shan(College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050000,China;HeBei Kingmoral biotech Co.,Ltd.,Shijiazhuang 050000,China;Innovation Center of food quality and safety testing technology of Hebei Province,Shinjiazhuang 051130,China)

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050000 [2]河北英茂生物科技有限公司,河北石家庄050000 [3]河北省食品质量与安全检测技术创新中心,河北石家庄051130

出　　处：《食品工业科技》2021年第12期398-404,共7页Science and Technology of Food Industry

基　　金：河北省高等学校科学技术研究项目(QN2020177);石家庄市科学技术研究与发展计划项目(201170283A);河北省青年科学基金项目(C2019208188);河北科技大学博士科研基金项目(81/1181288);河北科技大学博士科研基金项目(81/1181289);河北科技大学大学生创新创业项目(2020051)。

摘　　要：食品中真菌毒素污染成为近年来食品安全检测领域的热点和难点。免疫分析技术具有检测快速、操作简便、价格低廉且环境友好等优点,适合应用于食品安全检测领域。本文对基于新型标记材料的免疫分析技术,包括酶联免疫吸附法、免疫层析技术、电化学免疫传感技术、免疫芯片技术、基于免疫分析的流式微球技术和时间分辨荧光免疫分析技术,应用于粮食产品中真菌毒素快速检测的研究现状进行综述,系统分析了各种免疫分析技术的优缺点,为免疫分析技术在真菌毒素检测的应用及发展提供参考,同时也为保障粮食产品的安全提供了新思路。In recent years,mycotoxin contamination in food has become a hot and difficult issue in the field of food safety.Immunoassay has the advantages of short detection time,simple operation steps,low cost and environmental friendliness,which is suitable for food safety detection.The research of new labeling materials-based immunoassay techniques in rapid detection of mycotoxin in cereal products is reviewed in this paper,including enzyme linked immunosorbent assay,immunochromatography,electrochemical immunosensor,immunochips,cytometric bead array based on immunoassay and time resolved fluorescence immunoassay.The advantages and disadvantages of the above immunoassay are systematically analyzed,which provides references for the application and development of immunoassay techniques in the detection of mycotoxins,and also provides new ideas for ensuring safety of grain products.

关 键 词：真菌毒素 新型标记材料 酶联免疫吸附法 免疫层析技术 电化学免疫传感器 免疫芯片技术 基于免疫分析的流式微球技术 时间分辨荧光免疫分析技术 

分 类 号：TS207[轻工技术与工程—食品科学]