嗜麦芽窄食单胞菌的培养基优化及其在烟叶发酵中的初步应用研究  

Optimization of medium for Stenotrophomonas sp.and its preliminary application in tobacco fermentation

作　　者：黄申 周利峰 吕乔 孙海峰 景天 李石头[3] 闫茗熠 毛多斌 HUANG Shen;ZHOU Lifeng;LYU Qiao;SUN Haifeng;JING Tian;LI Shitou;YAN Mingyi;MAO Duobin(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China;Shenzhen Tobacco Industrial Co.,Ltd.,Shenzhen 518109,China;Technology Center,China Tobacco Zhejiang Industrial Co.,Ltd.,Hangzhou 310024,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,河南郑州450001 [2]深圳烟草工业有限责任公司,广东深圳518109 [3]浙江中烟工业有限责任公司技术中心,浙江杭州310024

出　　处：《轻工学报》2021年第3期36-44,共9页Journal of Light Industry

基　　金：烟草总公司重点科技研发项目(中烟办[2020]66号)。

摘　　要：采用单因素试验对嗜麦芽窄食单胞菌(Stenotrophomonas sp.H3-1)的培养基进行优化,在此基础上,将该菌株制备成菌制剂后初步应用于烟叶发酵,并对比研究发酵前后烟叶的品质变化.结果表明:优化后嗜麦芽窄食单胞菌培养基中适宜的碳源为质量浓度为2.0 g/L的麦芽糖,氮源为质量浓度为2.0 g/L的硫酸铵,初始pH值为8,培养温度为40℃;烟叶发酵后有5种香味物质的质量浓度发生了明显变化,其中对香气组成影响较大的茄酮和9-羟基-4,7-巨豆二烯-3-酮的质量浓度均有不同程度的增加;评吸结果表明,利用菌制剂发酵烟叶后,其香气质、香气量和香气浓度均有所提升.The medium of Stenotrophomonas sp.H3-1 was optimized by one single factor experiment.The strain was prepared into bacteria-preparation and preliminarily used in tobacco fermentation,and the quality changes of tobacco leaves before and after fermentation were compared.It showed that the optimized culture medium was 2.0 g/L of maltose asthe carbon source and the nitrogen source is ammonium sulfate of 2.0 g/L,initial pH value 8,temperature 40℃.After tobacco fermentation,the mass concentrations of five flavor substances changed obviously,the mass concentrations of solanone and 9-hydroxy-4,7-megadiene-3-one,which had greater effect on aroma composition,all increased to different degrees.The evaluation results showed that the aroma quality,aroma quantity and aroma concentration of tobacco leaves were improved after fermented with bacterial preparations.

关 键 词：嗜麦芽窄食单胞菌 培养基优化 烟叶发酵 香味物质 

分 类 号：TS444[农业科学—烟草工业] Q93[生物学—微生物学]