酶法脱乙酰化制备壳寡糖的研究进展  

Research Progress of Enzymatic Deacetylation to Make Chitosan Oligosaccharides

作　　者：周勇 ZHOU Yong(COFCO Biochemical Co.,Ltd.,Bengbu 233010,China)

机构地区：[1]中粮生物科技股份有限公司,安徽蚌埠233010

出　　处：《安徽农学通报》2021年第11期27-31,118,共6页Anhui Agricultural Science Bulletin

摘　　要：壳寡糖是甲壳素脱除乙酰基和降解后的低分子聚合物,因其具有黏度低、水溶性好、保湿性强、吸收能力强及生物相容性好等特性而具有广泛的应用价值。传统生产壳寡糖工艺存在能耗高、污染重且脱乙酰度不均匀等问题,而酶法制备壳寡糖能有效避免上述问题。当前,酶法制备壳寡糖的关键酶在于甲壳素脱乙酰酶。该文介绍了甲壳素脱乙酰酶的来源与酶学性质,结构与催化机理以及其生产菌株的选育,以期为后续产业化应用提供参考。Chitosan oligosaccharides(COS)is a kind of low molecular polymer after deacetylation and degradation of chitin.It has a wide range of application value because of its low viscosity,good water solubility,strong moisture re-tention,strong absorption capacity and good biocompatibility.The traditional COS preparation process has someproblems,such as high energy consumption,heavy pollution and uneven deacetylation degree,while the enzymatic method can effectively avoid the above problems.At present.chitin deacetylase is the key enzyme for enzymatic prep-aration of COS.This paper mainly introduces the source,enzymatic properties,structure and catalytic mechanism of chitin deacetylase,as well as the screening of its producing strains,in order to provide reference for the subsequent industrial application.

关 键 词：甲壳素 壳寡糖 甲壳素脱乙酰酶 脱乙酰 

分 类 号：TS201.2[轻工技术与工程—食品科学]