顶空固相微萃取气质联用法分析羊耳菊花的挥发性成分  

Volatile constituents in the flowers of Inula cappa based on HS-SPME-GC-MS

作　　者：许培增 刘明娟 罗贞贵 曾雅静 蔡伟 XU Pei-zeng;LIU Ming-juan;LUO Zhen-gui;ZENG Ya-jing;CAI Wei(Hunan University of Medicine,Huaihua 418000,Hunan,China;Hunan Province Key Laboratory for Antibody-based Drug and Intelligent Delivery System,Hunan University of Medicine,Huaihua 418000,Hunan,China;Hunan Provincial Key Laboratory of Dong Medicine,Hunan University of Medicine,Huaihua 418000,Hunan,China)

机构地区：[1]湖南医药学院,湖南怀化418000 [2]湖南医药学院新型抗体药物及其智能运输系统湖南省重点实验室,湖南怀化418000 [3]湖南医药学院侗医药研究湖南省重点实验室,湖南怀化418000

出　　处：《湖北农业科学》2021年第11期115-117,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(81603393);湖南省自然科学基金项目(2018JJ3376)。

摘　　要：利用顶空固相微萃取(HS-SPME)结合气相色谱质谱联用技术(GC-MS)分析羊耳菊(Inula cappa)花的挥发性化学成分,并采用归一化法计算各成分的相对含量。结果表明,从羊耳菊花中共检测鉴定出50种化合物,占总挥发性成分的86.74%。其主要成分为百里酚(19.70%)、(-)-β-石竹烯(12.19%)、香芹酚(8.13%)和异丁百里香酯(8.13%)。该方法具有操作简便、用量少、灵敏度高且快速等优点,研究结果为其药效物质基础研究和质量控制研究奠定基础。The volatile components in the flowers of Inula cappa were analyzed by headspace solid phase micro-extration(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS).The results showed that 50 compounds were identified,accounting for 86.74%of the total volatile components.Its main components include thymol(19.70%),(-)-β-caryophyllene(12.19%),carva⁃crol(8.13%)and thymyl isobutyrate(8.13%).This method can quickly obtain its components,is advantageous to volatile com⁃pounds,can be used for the control of Inula cappa.The method has the advantages of simple operation,low dosage,high sensitivity and high speed.The results of this study lay a foundation for the study of pharmacodynamics and quality control.

关 键 词：羊耳菊(Inula cappa)花 顶空固相微萃取 气相色谱-质谱联用 挥发性成分 保留指数 

分 类 号：R284.2[医药卫生—中药学]