超声波-酶法提取苤蓝花青素的工艺优化及体外抗氧化性  

Optimization of Ultrasonic Enzymatic Extraction Anthocyanin of Kohlrabi and Its Antioxidant Activity in Vitro

作　　者：赵孟良[1,2] 孙世英 任延靖 Zhao Mengliang;Sun Shiying;Ren Yanjing(Qinghai Key Laboratory of Vegetable Genetics and Physiology,Academy of Agriculture and Forest Science,Qinghai University,Xining,810016;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining,810016)

机构地区：[1]青海大学农林科学院,青海省蔬菜遗传与生理重点实验室,西宁810016 [2]青海大学,省部共建三江源生态和高原农牧业国家重点实验室,西宁810016

出　　处：《分子植物育种》2021年第11期3727-3736,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31960602);青海省蔬菜遗传与生理重点实验室项目(2020-ZJ-Y02);青海省科技厅青年基金项目(2019-ZJ-979Q);青海省农林科学院创新基金项目(2018-NKY-006;2018-NKY-008);青海大学农牧学院大学生科研训练项目(NKX201802)共同资助。

摘　　要：为研究苤蓝花青素提取工艺及其抗氧化性,本研究采取超声波辅助和酶辅助两种方法进行了提取和工艺优化,并对提取出的花青素进行抗氧化分析。在酶辅助法中通过单因素实验研究了料液比、水浴温度、酶用量、水浴提取时间对花青素提取率的影响;在超声法中研究了料液比、超声温度、超声时间对花青素提取率的影响,最终通过单因素实验筛选出的最优结果又通过三因素三水平的正交试验优化了两种提取工艺,并采用DPPH法对花青素进行抗氧化分析。结果表明,酶法最优提取工艺为料液比1∶25、纤维素酶用量2%、提取温度45℃、提取时间为90 min;超声法最优提取工艺为料液比1∶25,超声时间90 min,超声温度45℃;苤蓝中花青素具有极强的抗氧化性。本研究为今后苤蓝花青素的提取及开发利用提供了参考。In order to study the extraction process and antioxidant properties of kohlrabi anthocyanins,ultrasonic assisted and enzyme assisted methods were used to extract and optimize the process,and the antioxidant analysis of the extracted anthocyanins was carried out.The effects of feed-liquid ratio,water bath temperature,enzyme dosage and water bath extraction time on anthocyanin extraction rate were studied by enzyme assisted method.The effects of feed-liquid ratio,ultrasonic temperature and ultrasonic time on anthocyanin extraction rate were studied in ultrasonic method.Finally,the optimal results were selected by single factor experiment and two extraction processes were optimized by orthogonal test of three factors and three levels,and the conventional DPPH method was used to analyze the antioxidation of Brassica oleracea var.Caulorapa.The results showed that the optimum extraction process is 1∶25 ratio of material to liquid,2%amount of cellulase,extraction temperature 45℃,extraction time is 90 min;ultrasonic optimum extraction process is 1∶25 ratio of material to liquid,ultrasonic time is 90 min,ultrasonic temperature is 45℃;kohlrabi anthocyanins in blue have extremely strong oxidation resistance.This study provides a reference for the extraction and exploitation of kohlrabi anthocyanins in the future.

关 键 词：苤蓝 花青素 提取工艺 抗氧化性 

分 类 号：TQ28[化学工程—有机化工]