火麻籽多肽对高脂饮食喂养大鼠血脂的影响  

Effect of Hemp Seed Peptide on Blood Lipids in High-Fat Diet Fed Rats

作　　者：魏连会[1] 宋淑敏[1] 董艳[1] 石杰[1] 王娇娇 李宝龙 孙兴荣 WEI Lianhui;SONG Shumin;DONG Yan;SHI Jie;WANG Jiaojiao;LI Baolong;SUN Xingrong(Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,China;College of Basic Medical,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing 163316,China)

机构地区：[1]黑龙江省科学院大庆分院,黑龙江大庆163319 [2]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040 [3]黑龙江省农业科学院大庆分院,黑龙江大庆163316

出　　处：《食品科学》2021年第11期161-167,共7页Food Science

基　　金：黑龙江省院所基本应用技术研究专项(ZNBZ2018DQ04);黑龙江省科学院招标项目(ZB2018004);黑龙江省科学院学科领域创新能力提升专项(XKLY2019DQ01)。

摘　　要：目的:通过动物实验对火麻籽多肽进行降血脂功能性进行研究。方法:选取60只SD雄性大鼠,适应性饲养1周后,分成正常对照组(10只)、模型组(50只),正常对照组给予基础饲料,模型组饲喂高脂饲料,连续饲喂4周建立高血脂模型大鼠;造模成功后将模型组平均分为高脂模型组、阳性对照组(辛伐他汀,10 mg/kg mb)和火麻籽多肽低(200 mg/kg mb)、中(400 mg/kg mb)、高(800 mg/kg mb)剂量组,分别经口灌胃饲喂4周后测定大鼠体质量,脏器指数,血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine aminotransferase,ALT)水平,血液流变学变化,肝脏病理切片组织形态学变化。结果表明:与高脂模型组相比,火麻籽多肽能够抑制大鼠的体质量增长,降低肝脏指数和血清中TC、TG、LDL-C、AST、ALT水平,升高HDL-C水平,降低动脉硬化指数,改善大鼠血流变学指标,降低大鼠的全血黏度和血浆黏度;改善肝脏脂肪变性情况。结论:火麻籽多肽对高脂饮食喂养大鼠具有一定的降脂作用。The hypolipidemic activity of hemp seed peptide was investigated by animal tests.Sixty male SD rats were randomly divided into the normal group with 10 rats and the model group with 50 rats after one week of adaptive feeding.The rats in the normal group were fed a basic diet and the rats in the model group were fed a high-fat diet(HFD)to induce hyperlipidemia for four weeks of feeding.After successful modeling,the model rats were randomly divided into model control,positive control(simvastatin at 10 mg/kg mb),and low-,medium-,and high-dose hemp seed peptide groups(200,400,and 800 mg/kg mb)with 10 animals each.The route of administration was by intragastric gavage.After 4 weeks of feeding,body mass,visceral organ index,blood rheology,and serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were measured,and morphological changes in the liver of the rats were observed.The results obtained showed that hemp seed peptide could inhibit body mass gain in high-fat diet-fed rats,reduce serum TC,TG,LDL-C,ALT and AST and liver index and atherosclerosis index(AI)and augment serum HDL-C.In addition,it could improve blood rheology such as reducing whole blood viscosity and plasma viscosity and the fatty degeneration of the liver.In conclusion,hemp seed peptide has a lipid-lowering effect in HFD rats.

关 键 词：火麻籽多肽 高脂血症 降血脂作用 

分 类 号：TS201.4[轻工技术与工程—食品科学]