酵母菌代谢产物对植物乳杆菌AB-1生产苯乳酸的影响  

Effect of Yeast Metabolites on the Production of Phenyllactic Acid by Lactobacillus plantarum AB-1

作　　者：张成林 钱怡霖 刘尊英[1] ZHANG Cheng-lin;QIAN Yi-lin;LIU Zun-ying(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandong,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品研究与开发》2021年第11期145-151,共7页Food Research and Development

基　　金：国家自然科学基金(31972141)。

摘　　要：以植物乳杆菌AB-1(Lactobacillus plantarum AB-1)为研究对象,探究不同培养基组成以及外源添加酵母菌无细胞发酵上清液(cell-free supernatant,CFS)对植物乳杆菌AB-1生产苯乳酸的影响。结果表明,将培养植物乳杆菌AB-1的MRS培养基中葡萄糖浓度由20.0 g/L提高至27.5 g/L、蛋白胨浓度由10.0 g/L提高至30.0 g/L时,苯乳酸产量从81.2 mg/L提高至103.8 mg/L,提高了27.8%(P<0.05)。在此基础上,外源添加15%经40 h发酵的酵母菌E1 CFS,可使苯乳酸产量达到130.2 mg/L,相较于优化MRS培养基和初始MRS培养基分别提高了25.4%和60.3%(P<0.05)。进一步研究表明,植物乳杆菌AB-1苯乳酸产量的提高可能与酵母菌CFS中苯丙氨酸(Phe)和α-酮戊二酸(α-KG)两种代谢产物有关。综上,该研究通过优化培养基及外源添加酵母菌CFS的方法,找到一种提高植物乳杆菌AB-1生产苯乳酸能力的培养方式。The effects on the production of phenyllactic acid(PLA)by Lactobacillus plantarum AB-1 were in-vestigated by optimizing the medium composition and adding yeast cell-free supernatant(CFS).The results in-dicated that the production of PLA by L.plantarum AB-1 increased from 81.2 mg/L to 103.8 mg/L in a modified MRS medium in which the concentration of glucose increased from 20.0 g/L to 27.5 g/L and the concentration of peptone increased from 10.0 g/L to 30.0 g/L,separately.Additionally,after adding 15%40 h-fermented yeast E1 cell-free fermentation supernatant,the production of PLA by L.plantarum AB-1 significantly increased to 130.2 mg/L,an enhancement of 60.3%and 25.4%compared with that under the original and optimal MRS cul-ture conditions,respectively.Further investigation revealed that the increase in PLA production may be associ-ated with the presence of phenylalanine andα-ketoglutarate in the yeast cell-free fermentation supernatant.In conclusion,this study proposes a feasible method to increase the production of PLA by L.plantarum AB-1.

关 键 词：乳酸菌 发酵 苯乳酸 酵母菌代谢产物 生物防腐剂 

分 类 号：TS201.3[轻工技术与工程—食品科学]