直投式发酵剂制备过程中发酵乳杆菌LF-8001固定化技术研究  

Study on immobilization technology of Lactobacillus fermentum strain LF-8001

作　　者：刘林新 王修俊 张二康[1,2] 于沛 杨丽平 陈颜红 LIU Linxin;WANG Xiujun;ZHANG Erkang;YU Pei;YANG Liping;CHENG Yanhong(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;Guizhou Provincial Key Laboratory of Fermentation Engineering And Biopharmacy Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵阳550025 [2]贵州省发酵工程与生物制药重点实验室,贵阳550025

出　　处：《包装与食品机械》2021年第3期15-20,共6页Packaging and Food Machinery

基　　金：贵州省科技计划项目(2016-2357)。

摘　　要：为了研究从自然发酵泡菜中筛选出的一株有高效降解亚硝酸盐(NIT)功能的发酵乳杆菌LF-8001的固定化技术,利用发酵乳杆菌LF-8001浓缩菌液,改变氯化钙浓度、固定化温度及混合比例,以包埋率、菌种活力和NIT降解率为指标,结合固定化小球物理参数,进行正交试验。结果表明,当固定化液:菌液为1:4,温度为25℃、氯化钙为4%时,发酵乳杆菌LF-8001固定化小球的NIT降解率达到91.23%,活菌数达到1.49×10^(11) CFU/g。该研究技术可制成富含菌体的固定化小球,为发酵蔬菜发酵乳杆菌直投式发酵剂制备提供有力支撑。In order to study the immobilized pellet technology of a strain of Lactobacillus fermentum LF-8001 that has the function of efficiently degrading nitrite(NIT)selected from natural fermented kimchi,the concentration of Lactobacillus fermentum LF-8001 with high degrading NIT content is used.For the bacterial solution,the concentration of calcium chloride,the immobilization temperature and the mixing ratio were changed,and the embedding rate,the vitality of the bacteria and the degradation rate of NIT were used as indicators,combined with the physical parameters of the immobilized pellets,to conduct an orthogonal experiment.The results showed that when the ratio of immobilized liquid:bacterial liquid was 1:4,the temperature was 25℃,and calcium chloride was 4%,the degradation rate of nitrite in the immobilized pellets of Lactobacillus fermentum LF-8001 reached 91.23%,and the bacterial concentration It is 1.49×10^(11) CFU/g.The research technology can be made into immobilized pellets rich in bacteria,which provides strong support for the preparation of direct-investment starter for fermented vegetable Lactobacillus fermentum.

关 键 词：固定化技术 直投式发酵剂 发酵乳杆菌 发酵蔬菜 

分 类 号：TS255.54[轻工技术与工程—农产品加工及贮藏工程]