优化QuEChERS结合LC-MS-MS法测定水产品中8种多环芳烃的研究  

Determination of 8 Polycyclic Aromatic Hydrocarbons in Aquatic Products by LC-MS-MS Coupled with Optimized QuEChERS

作　　者：房翠兰[2] 王贵敏 向利 肖伦[2] 冉秀芝[1] FANG Cui-lan;WANG Gui-min;XIANG Li;XIAO Lun;RAN Xiu-zhi(College of Chemistry and Chemical Engineering,Chongqing University of Technology,Chongqing 400054,China;Chongqing Jiulongpo District Center for Disease Control and Prevention,Chongqing 400039,China)

机构地区：[1]重庆理工大学化学化工学院,重庆400054 [2]重庆市九龙坡区疾病预防控制中心,重庆400039

出　　处：《食品与发酵科技》2021年第3期152-156,163,共6页Food and Fermentation Science & Technology

基　　金：重庆市九龙坡区科技局社会事业类项目(项目编号:社201808)。

摘　　要：以市售常见的水产品为研究对象,建立并优化了QuEChERS结合LC-MS-MS测定水产品中8种多环芳烃残留量的方法。样品经超声辅助乙腈提取,经C18和弗洛里硅土双连接层析柱净化后,采用液相色谱-质谱检测器对多环芳烃进行检测分析。结果表明,8种多环芳烃分离效果好,检出限(LOD)为0.006~0.105μg/kg,相对标准偏差为0.3%~4.0%(n=4),加标回收率达到86%~110%。这表明该方法具有比较高的重现性和选择性,对于水产品中多环芳烃的残留测定具有很好的应用价值。By taking the common aquatic products on the market as material,an analytical method for the determination of 8 polycyclic aromatic hydrocarbons(PAHs)in aquatic products by liquid chromatography-mass spectrometry was established.The sample was extracted by ultrasonic assisted acetonitrile,and the extract was cleaned up with C_(18 ) and Florisil chromatographic bi-column,detected with LC-MS/MS for PAHs.The results showed that the 8 PAHs could be separated efficiently.The detection limits of this method were 0.006~0.105μg/kg.The relative standard deviations were ranging from 0.3%~4.0%(n=4),and the recovery rates were from 86%~110%.The method proposed by this paper possesses higher producibility and selectivity,and has promising applications in determining the PAHs in aquatic products.

关 键 词：QUECHERS 多环芳烃 液相色谱-质谱 水产品 

分 类 号：TS207.3[轻工技术与工程—食品科学]