中长链甘油三酯对大鼠肝脏脂质代谢的调节作用  

Regulation of Medium and Long-Chain Triacylglycerols on the Lipid Metabolism in Rat Livers

作　　者：杜映雪 李静[1] 陈孙妮 李娟 邓泽元[1] Du Yingxue;Li Jing;Chen Sunni;Li Juan;Deng Zeyuan(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《中国食品学报》2021年第6期114-122,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2016YFD040140402)。

摘　　要：为研究中长链甘油三酯(MLCT)在短期内对大鼠肝脏脂质组成及脂代谢的影响,选取雄性SD大鼠随机分为空白对照组、中长链甘油三酯组(MLCT组)、中链甘油三酯组(MCT组)、高油酸菜籽油组(HORO组),每日以1g/100g体重的剂量灌胃相应脂质2周。结果显示:与HORO组相比,MLCT显著降低大鼠血浆中甘油三酯(TG)和总胆固醇(TC)的含量,且降低低密度脂蛋白胆固醇(LDL-C)的含量;MLCT显著抑制大鼠肝脏质量的增加,降低肝脏中TC、TG含量,使载脂蛋白A1/载脂蛋白B比值显著性升高。各组大鼠肝脏中甘油三酯、磷脂(PL)的脂肪酸组成变化显著,说明摄入不同脂质可导致肝脏脂肪酸组成的改变。MCT组大鼠肝脏中激素敏感性脂肪酶(HSL)、环磷酸腺苷(cAMP)和蛋白激酶A(PKA)水平最高,脂肪甘油三酯脂酶(ATGL)水平最低。相反,HORO组的HSL、cAMP和PKA水平最低,而ATGL水平最高。MLCT组上述脂质代谢相关酶水平均介于MCT、HORO组之间,且均高于对照组。本研究表明,MLCT在2周内可有效改善大鼠体内脂质代谢,提高肝脏中脂质代谢的效率,从而降低脂代谢以及相关疾病的发生风险。This study aims to investigate the effects of medium and long-chain triacylglycerols(MLCT)on lipid composition and lipid metabolism in rat livers in the short term.Male SD rats were randomly divided into Control group,MLCT group,MCT group and HORO group.The rats were fed with oil at the dose of 1 g/100 g body weight per day for 2 weeks.The results showed that compared with HORO group,MLCT significantly decreased the contents of triacylglycerol(TG)and total cholesterol(TC)in plasma,and also decreased low density lipoprotein-cholesterol(LDL-C).MLCT significantly inhibited the increase of liver weight,reduce the contents of TC and TG in livers,and significantly increase the ratio of apolipoprotein A1/apolipoprotein B.The fatty acid compositions of triacylglycerols and phospholipids in the livers of rats in each group changed greatly,suggesting that the intake of different lipids would lead to changes in the fatty acid of the liver.In MCT group,the levels of hormone-sensitive triglyceride lipase(HSL),cyclic adenosine monophosphate(cAMP)and protein kinase A(PKA)were the highest,while the levels of adipose triacylglyceride lipase(ATGL)were the lowest.On the contrary,the levels of HSL,cAMP and PKA in HORO group were the lowest,while the levels of ATGL in HORO group were the highest.The levels of lipid metabolism related enzymes in MLCT group were between that of MCT group and HORO group,and were higher than those in Control group.This study suggested that within two weeks,MLCT could improve lipid metabolism in rats,increase the efficiency of lipid metabolism in livers,and reduce the risk of lipid metabolism and its related diseases.

关 键 词：中长链甘油三酯 肝脏 脂质代谢 脂肪酸 

分 类 号：TS201.4[轻工技术与工程—食品科学]