Visualization of perforin/gasdermin/complement-formed pores in real cell membranes using atomic force microscopy  

作　　者：Yuying Liu Tianzhen Zhang Yabo Zhou Jiping Li Xiaoyu Liang Nannan Zhou Jiadi Lv Jing Xie Feiran Cheng Yiliang Fang Yunfeng Gao Ning Wang Bo Huang 

机构地区：[1]Department of Immunology&National Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,School of Basic Medicine Peking Union Medical College,Beijing 100005,China [2]Clinical Immunology Center,Chinese Academy of Medical Sciences,Beijing 100005,China [3]Beijing SOJO Electric Company Limited,Beijing,China [4]Department of Mechanical Science and Engineering,College of Engineering,University of Illinois at Urbana-Champaign,Urbana,Illinois 61801,USA [5]Laboratory for Cellular Biomechanics and Regenerative Medicine,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan,Hubei 430074,China [6]Department of Biochemistry&Molecular Biology,Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430030,China

出　　处：《Cellular & Molecular Immunology》2019年第6期611-620,共10页中国免疫学杂志（英文版）

基　　金：supported by grants from Chinese Academy of Medical Sciences(CAMS)Initiative for Innovative Medicine(CAMS-I2M)2017-I2M-1-001,the National Natural Science Foundation of China(81788101,81661128007,81530080,and 81773062);the CAMS Initiative for Innovative Medicine(2016-I2M-1–007).

摘　　要：Different types of pores ubiquitously form in cell membranes,leading to various types of cell death that profoundly influence the fate of inflammation and the disease status.However,these pores have never truly been visualized to date.Atomic force microscopy(AFM),which is emerging as a powerful tool to analyze the mechanical properties of biomolecules and cells,is actually an excellent imaging platform that allows biological samples to be visualized by probing surface roughness at the level of atomic resolution.Here,membrane pore structures were clearly visualized using AFM.This visualization not only describes the aperture and depth of the pore complexes but also highlights differences among the pores formed by perforin and gasdermins in tumor cell membranes and by complement in immune cell membranes.Additionally,this type of visualization also reveals the dynamic process of pore formation,fusion,and repair.

关 键 词：membrane pore formation AFM PERFORIN GSDMD/GSDME COMPLEMENT 

分 类 号：R73[医药卫生—肿瘤]